Studies on the Ultrasonication Parameters in Pollen-mediated Genetic Transformation of Grape

doi:10.3321/j.issn:1000-7091.2006.01.003

Abstract

Abstract: Grape pollens were treated by pollen-mediated ultrasonication additionally,the results indicated that each parameter of ultrasonication had significant effect on grape pollen,furthermore,significant interaction were detected between parameters.Therefore,it is suggested that when treating pollens by ultrasonication and choosing suitable parameters,the interaction should be considered.According to significant test results,stability of the pollen broken rate and the germinated rate,on the basis of non-destructive DNA,the best choosing range of ultrasonication parameter were:the power in 100-120 W,treating time in 4-5 s,interval time in 2-6 s,and 6 times of treat number.The GUS activity measuring result showed that the transformation ratios of the pollen was 4‰.

Key words: Grape, Pollen mediated, Genetic transformation

CLC Number:

S663.1

YUE Xin-li, CAO Qiu-fen, JI Lan, MENG Yu-ping, CHEN Jun. Studies on the Ultrasonication Parameters in Pollen-mediated Genetic Transformation of Grape[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(1): 10-13. doi: 10.3321/j.issn:1000-7091.2006.01.003.

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