Rye Genomic Specific SCAR Marker Detected Leaf Rust Resistance Related Gene in Rye Transferred to Wheat

doi:10.3969/j.issn.1000-7091.2013.03.034

Abstract

Abstract: A SCAR marker of rye genome was used to amplified the TcLr45 and the background material Thatcher． One unique band in TcLr45，and two different bands in Thatcher were implified． In addition， 49 wheat leaf rust resistance near isogenic gene lines( NILs) were detected by the marker， the marker is specific for TcLr45 among these genes． F2 segregate groups screening results showed a long distance genetic linkage between Lr45 and the marker． The band was cloned and sequenced， the full length 650 bp，which was different from the sequence amplified in rye ( 643 bp) ，compared the two sequences by BLAST2 soft， the homology is 97%． 16 of 100 wheat varieties were amplified the same bands as TcLr45

Key words: Rye, Leaf Rust resistance gene, Lr45, SCAR marker

CLC Number:

Liu Chunyan, GUO Nan, Yan Hongfei, Liu Daqun. Rye Genomic Specific SCAR Marker Detected Leaf Rust Resistance Related Gene in Rye Transferred to Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(3): 189-192. doi: 10.3969/j.issn.1000-7091.2013.03.034.

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