Determination of Resistance Genotypes with STS Markers in Current Wheat Cultivars

doi:10.3969/j.issn.1000-7091.2013.03.033

Abstract

Abstract: In this study， two markers Vp1A3 and Vp1B3 associated with PHS tolerance were used to evaluate the 46 white-grained wheats， in order to select out the cultivar which had the strong capacity of resistance PHS tolerance，and amplify these resistance PHS cultivars in wheat production and breeding for resistance PHS． In this study，total thirteen Vp-1A haplotypes，which were Vp-1Aam，Vp-1Aan，Vp-1Aao，Vp-1Abm，Vp-1Abn，Vp-1Agm，Vp-1Agn，Vp-1Ahm，Vp-1Aho，Vp-1Aim，Vp-1Ain，Vp-1Aio and Vp-1Ajm with frequencies of 17.4%，8.7%，2.2%，4.3%，4.3%，6.5%，2.2%，2.2%，2.2%， 15.2%， 28.3%，4.3% and 2.2% respectively，were detected by STS marker Vp1A3，and three Vp-1B haplotypes，which were Vp-1Bc，Vp-1Ba and Vp-1Bb with frequencies of 71.7%，17.4%and 4.3%， respectively，were detected by STS marker Vp1B3． In addition， there were five cultivars were selected out，which were Mianyang 31，Yumai 35，Fengmai 24，CA0489 and Shandong 928802，having the gene about resistance PHS． The haplotypes of Vp-1 in these five cultivars，Mianyang 31，Yumai 35，Fengmai 24，CA0489 and Shandong 928802，were Vp-1Agm and Vp-1Bc，Vp-1Agm and Vp-1Bc，Vp-1Agm and Vp-1Bc，Vp-1Aam and Vp-1Bb，Vp-1Abm and Vp-1Bb， respectively． Therefore， selecting efficiency for seeds dormancy could be improved by using a combination of the STS markers Vp1A3 and Vp1B3．

Key words: Wheat, Pre-harvest sprouting, Molecular marker, Vp1A3, Vp1B3

CLC Number:

S512.03

Yang Yan, Wang Xiaoli1, Liu Shixin1, Zhao Xianlin. Determination of Resistance Genotypes with STS Markers in Current Wheat Cultivars[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(3): 183-188. doi: 10.3969/j.issn.1000-7091.2013.03.033.

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