Effects of Salinity-alkalinity Stress on Rhizosphere Soil Microbial Quantity and Enzyme Activity of Common Bean

doi:10.7668/hbnxb.201751393

Abstract

Abstract: A pot experiment was conducted to study the effects of salinity-alkalinity stress on rhizosphere microecological environment of two common bean varieties, HYD and JW. Two kinds of alkaline salts(Na₂CO₃, NaHCO₃) were mixed together in the proportion of 1:9. According to the percentage of soil mass after mixing, four treatments of 0(S0), 0.4%(S1), 0.8%(S2)and 1.2%(S3)were set to investigate the effects of different treatments on rhizosphere soil microbial quantity and enzyme activity. The results showed that under salt-alkali stress, HYD was better than JW in plant height, root length, shoot biomass and root biomass. There was a significant difference in the number of bacteria, fungi and actinomycetes in rhizosphere soil under saline-alkali stress treatments, and the number of bacteria was higher than those of fungi and actinomycetes. The soil urease, phosphatase, catalase activity and soil microbial quantity in rhizosphere of two common bean cultivars were the maximum in S1 treatment, reaching to a significant difference. Under the same treatment, the growth characteristics of plant height, root length,shoot biomass and root biomass of HYD were significantly higher than those of JW. The number of microorganisms and enzyme activity in the rhizosphere soil of HYD were significantly higher than those of JW. Correlation analysis showed that urease activity had significantly or extremely significantly positive correlation with the numbers of bacteria, fungi and actinomycetes, and total amount of microorganisms, while there was significantly positive correlation between phosphatase activity and the number of actinomycetes and between catalase activity and the number of fungi. Under salinity-alkalinity stress, the soil pH rose and microbial population changed, which led to changes in enzyme activity and indirected changes in rhizosphere soil microenvironment. A certain amount of salinity-alkalinity stress is beneficial to the improvement of soil microorganism and enzyme activity in common bean rhizosphere. A certain amount of saline-alkali stress promotes the growth of common bean.

Key words: Common bean, Salinity-alkalinity stress, Rhizosphere, Microbial quantity, Soil enzyme

CLC Number:

S144

GUO Xiaoxiao, WANG Xuelai, LIANG Haiyun, YU Song, YU Lihe, GUO Wei, FANG Mengying, ZHENG Lina, LI Xin. Effects of Salinity-alkalinity Stress on Rhizosphere Soil Microbial Quantity and Enzyme Activity of Common Bean[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(4): 148-157. doi: 10.7668/hbnxb.201751393.

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