Effects of Different Concentrations of Melatonin on Cadmium Accumulation of Nasturtium officinale

doi:10.7668/hbnxb.201751500

Abstract

Abstract: In order to improve the resistance of Nasturtium officinale to cadmium(5 mg/kg)stress and reduce its cadmium content, the pot experiment was conducted to study the effects of different concentrations of melatonin(0, 25, 50, 100, 150, 200 μmol/kg)on the biomass, cadmium content, photosynthetic pigment content, antioxidant enzyme activity of N. officinale, and enzyme activity, pH value and available cadmium content of soil. The results showed that melatonin effectively increased the superoxide dismutase, peroxidase, catalase activities of N. officinale leaves compared with the control, and all increased with the increase of melatonin concentration. The biomasses of roots, stems and leaves, and leaf photosynthetic pigment(chlorophyll a, chlorophyll b, carotenoid)content of N. officinale increased first and then decreased with the increase of melatonin concentration, and reached the maximum when melatonin concentration was 50 μmol/kg. The cadmium contents in roots, stems and leaves of N. officinale decreased first and then increased with the increase of melatonin concentration, and were the lowest when the concentration of melatonin was 50 μmol/kg, which were 9.82%, 7.42% and 8.19%, respectively, lower than control. In addition, compared with the control, melatonin significantly increased the soil available cadmium content and soil invertase activity after planting N. officinale, and presented an increased trend with the increase of melatonin concentration, while the soil pH value decreased with the increase of melatonin concentration. In conclusion, the appropriate concentration of melatonin could effectively reduce the cadmium absorption of N. officinale and improve the resistance of N. officinale to cadmium stress. Melatonin concentration of 50 μmol/kg was the best, which could provide a reference for the cultivation and production of N. officinale in cadmium contaminated areas.

Key words: Nasturtium officinale, Melatonin, Cadmium, Growth

CLC Number:

HUANG Kewen, LIN Lijin, WANG Jun, LIU Ji, LIU Lei, WANG Ting, LIAO Mingan, REN Wei. Effects of Different Concentrations of Melatonin on Cadmium Accumulation of Nasturtium officinale[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(4): 140-147. doi: 10.7668/hbnxb.201751500.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.201751500

http://www.hbnxb.net/EN/Y2019/V34/I4/140

References

[1] 苗亚琼, 林清. 广西土壤重金属镉污染及对人体健康的危害[J]. 环境与可持续发展, 2016, 41(5):170-173. doi:10.3969/j.issn.(673-288X.2016.05.046). Miao Y Q, Lin Q. Pollution caused by heavy metal cadmium to the soil in Guangxi and its harm to human health[J]. Environment and Sustainable Development, 2016, 41(5):170-173.
[2] 张连科, 李海鹏, 黄学敏, 李玉梅, 焦坤灵, 孙鹏, 王维大. 包头某铝厂周边土壤重金属的空间分布及来源解析[J]. 环境科学, 2016, 37(3):1139-1146. doi:10.13227/j.hjkx.2016.03.045.
Zang L K, Li H P, Huang X M, Li Y M, Jiao K L, Sun P, Wang W D. Soil heavy metal spatial distribution and source analysis around an aluminum plant in baotou[J]. Environmental Science, 2016, 37(3):1139-1146.
[3] 欧阳燕莎, 刘爱玉, 李瑞莲. 镉对作物的影响及作物对镉毒害响应研究进展[J]. 作物研究, 2016, 30(1):105-110. doi:10.16848/j.cnki.issn.1001-5280.2016.01.027.
Oyang Y S, Liu A Y, Li R L. Research progress on effects of cadmium on crops and the response of crops to cadmium[J]. Crop Research, 2016,30(1):105-110.
[4] 李婧, 周艳文, 陈森, 高小杰. 我国土壤镉污染现状、危害及其治理方法综述[J]. 安徽农学通报, 2015, 21(24):104-107. doi:10.3969/j.issn.1007-7731.2015.24.044.
L J, Zhou Y W, Chen S, Gao X J. Actualities, damage and management of soil cadmium pollution in China[J]. Anhui Agricultural Science Bulletin, 2015, 21(24):104-107.
[5] Asgher M, Khan M R, Anjum N A, Khan N A. Minimising toxicity of cadmium in plants-role of plant growth regulators[J].Protoplasma,2015,252(2):399-413. doi:10.1007/s00709-014-0710-4.
[6] Shahid M, Dumat C, Khalid S, Niazi N K, Antunes P M C. Cadmium bioavailability, uptake, toxicity and detoxification in soil-plant system[J]. Reviews of Environmental Contamination and Toxicology, 2017, 241:73-137. doi:10.1007/398_2016_8.
[7] 李玉双, 胡晓钧, 孙铁珩, 侯永侠, 宋雪英, 杨继松, 陈红亮. 污染土壤淋洗修复技术研究进展[J]. 生态学杂志, 2011, 30(3):592-602. doi:10.13292/j.1000-4890.2011.0077.
L Y S, Hu X J, Sun T H, Hou Y X, Song X Y, Yang J S, Chen H L. Soil washing/flushing of contaminated soil a review[J]. Chinese Journal of Ecology, 2011, 30(3):592-602.
[8] Zhang Y W, Huang T, Huang X, Faheem M A, Yu L, Jiao B Q, Yin G Z, Shiau Y C, Li D W.Study on electro-kinetic remediation of heavy metals in municipal solid waste incineration fly ash with a three-dimensional electrode[J].RSC Advances,2017,7(45):27846-27852.doi:10.1039/c7ra01327b.
[9] 侯李云, 曾希柏, 张杨珠. 客土改良技术及其在砷污染土壤修复中的应用展望[J]. 中国生态农业学报, 2015, 23(1):20-26.doi:10.13930/j.cnki.cjea.140797.
Hu L Y, Zeng X B, Zhang Y Z. Application and outlook of alien earth soil-improving technology in arsenic-contaminated soil remediation[J]. Chinese Journal of Eco-agriculture, 2015, 23(1):20-26.
[10] Singh J, Kalamdhad A S.Effect of lime on speciation of heavy metals during composting of water hyacinth[J].Frontiers of Environmental Science and Engineering, 2016,10(1):93-102.doi:10.1007/s11783-014-0704-7.
[11] He K, Chen Y C, Tang Z H, Hu Y Y. Removal of heavy metal ions from aqueous solution by zeolite synthesized from fly ash[J].Environmental Science and Pollution Research,2016,23(3):2778-2788. doi:10.1007/s11356-015-5422-6.
[12] 毕冬雪, 邓亚娟, 孟凡德, 韦婧, 王海龙, 袁国栋. 腐殖质纳米颗粒对镉污染土壤的修复[J]. 环境工程学报, 2018, 12(5):1295-1302. doi:10.12030/j.cjee.201711218.
B D X, Deng Y J, Meng F D, Wei J, Wang H L, Yuan G D. Humic nanoparticles for remediation of Cd-contaminated soils[J]. Chinese Journal of Environmental Engineering, 2018, 12(5):1295-1302.
[13] Mahar A, Wang P, Ali A, Awasthi M K, Lahori A H, Wang Q, Li R H, Zhang Z Q. Challenges and opportunities in the phytoremediation of heavy metals contaminated soils:A review[J].Ecotoxicology and Environmental Safety,2016,126:111-121.doi:10.1016/j.ecoenv.2015.12.023.
[14] 张彩丽. 微生物修复重金属污染土壤的研究进展[J]. 安徽农业科学, 2015, 43(16):225-229. doi:10.13989/j.cnki.0517-6611.2015.16.080.
Zang C L. Research process on treatment of heavy metal contaminated soil by microbial remediation[J]. Journal of Anhui Agricultural Sciences, 2015, 43(16):225-229.
[15] 王蕊, 杨小龙, 须晖, 李天来. 高等植物褪黑素的合成和代谢研究进展[J]. 植物生理学报, 2016, 52(5):615-627. doi:10.13592/j.cnki.ppj.2016.0052.
Wng R, Yang X L, Xu H, Li T L. Research progress of melatonin biosynthesis and metabolism in higher plants[J]. Plant Physiology Journal, 2016, 52(5):615-627.
[16] Sharif R, Xie C, Zhang H Q, Arnao M B, Ali M, Ali Q, Muhammad I, Shalmani A, Nawaz M A, Chen P, Li Y H. Melatonin and its effects on plant systems[J].Molecules,2018,23(9):2352-2371. doi:10.3390/molecules23092352.
[17] 唐懿, 任纬, 刘副刚, 李焕秀, 廖明安, 蒋伟, 林立金. 褪黑素浸种对豌豆幼苗生长及镉积累的影响[J]. 土壤, 2018, 50(1):109-114. doi:10.13758/j.cnki.tr.2018.01.015.
Tng Y, Ren W, Liu F G, Li H X, Liao M A, Jiang W, Lin L J. Effects of melatonin soaking on growth and cadmium accumulation of pea seedlings[J]. Soils, 2018, 50(1):109-114.
[18] 刘自力, 黄一凡, 朱正波, 陈亚华, 崔瑾. 叶面喷施褪黑素对小白菜幼苗镉耐性的影响[J]. 植物生理学报, 2018, 54(4):660-668. doi:10.13592/j.cnki.ppj.2018.0066.
Lu Z L, Huang Y F, Zhu Z B, Chen Y H, Cui J. Effects of foliar feeding of melatonin on cadmium tolerance of Chinese cabbage seedlings[J]. Plant Physiology Journal, 2018, 54(4):660-668.
[19] 刘仕翔, 黄益宗, 罗泽娇, 黄永春, 杨秀文. 褪黑素对水稻镉积累及其化学结合形态的影响[J]. 应用生态学报, 2017, 28(5):1588-1594. doi:10.13287/j.1001-9332.201705.027.
Lu S X, Huang Y Z, Luo Z J, Huang Y C, Yang X W. Effects of exogenous melatonin on accumulation and chemical form of Cd in rice[J]. Chinese Journal of Applied Ecology, 2017, 28(5):1588-1594.
[20] Zhan F D, Qin L, Guo X H, Tan J B, Liu N N, Zu Y Q, Li Y. Cadmium and lead accumulation and low-molecular-weight organic acids secreted by roots in an intercropping of a cadmium accumulator sonchus asper l. with Vicia faba L.[J]. RSC Advances, 2016, 6(40):33240-33248. doi:10.1039/c5ra26601g.
[21] Pinela J, Barreira J C, Barros L, Verde S C, Antonio A L, Carvalho A M, Oliveira M B, Ferreira I C. Suitability of gamma irradiation for preserving fresh-cut watercress quality during cold storage[J].Food Chemistry,2016,206:50-58.doi:10.1016/j.foodchem.2016.03.050.
[22] 宋关玲. 铜和镉对水体修复植物西洋菜的生理影响[J]. 东北林业大学学报, 2008, 36(2):42-44. doi:10.13759/j.cnki.dlxb.2008.02.011.
Sng G L. Physiological effects of copper and cadmium on Nasturtium officinale R.Br[J]. Journal of North-East Forestry University, 2008, 36(2):42-44.
[23] 鲍士旦. 土壤农化分析[M]. 北京:中国农业出版社, 2000.
Bo S D. Soil and agricultural chemistry analysis[M]. Beijing:China Agriculture Press,2000.
[24] 李合生. 植物生理生化实验原理和技术[M]. 北京:高等教育出版社, 2000.
L H S. Principles and techniques of plant physiological and biochemical experiments[M]. Beijing:Higher Education Press,2000.
[25] Rastmanesh F, Moore F, Keshavarzi B. Speciation phytoavailability of heavy metals in contaminated soils in sarcheshmeh area, Kerman province,Iran[J].Bulletin of Environmental Contamination and Toxicology,2010,85(5):515-519. doi:10.1007/s00128-010-0149-z.
[26] Arnao M B, Hernández-Ruiz J. Functions of melatonin in plants:A Review[J]. Journal of Pineal Research, 2015, 59(2):133-150. doi:10.1111/jpi.12253.
[27] Chen Q, Qi W B, Reiter R J, Wei W, Wang B M. Exogenously applied melatonin stimulates root growth and raises endogenous indoleacetic acid in roots of etiolated seedlings of Brassica juncea[J].Journal of Plant Physiology,2009,166(3):324-328.doi:10.1016/j.jplph.2008.06.002.
[28] Kolá J, Machá ková I. Melatonin in higher plants:occurrence and possible functions[J].Journal of Pineal Research,2005,39(4):333-341.doi:10.1111/j.1600-079X.2005.00276.x.
[29] 姜超强, 祖朝龙. 褪黑素与植物抗逆性研究进展[J]. 生物技术通报, 2015, 31(4):47-55. doi:10.13560/j.cnki.biotech.bull.1985.2015.03.013.
Jang C Q, Zu C L. Advances in melatonin and its roles in abiotic stress resistance in plants[J]. Biotechnology Bulletin, 2015, 31(4):47-55.
[30] 李合生. 现代植物生理学[M]. 3版. 北京:高等教育出版社, 2012.
L H S. Modern plant physiology[M]. 3rd. Beijing:Higher Education Press,2012.
[31] 李佩华. 铅,镉胁迫对马铃薯叶绿素含量及细胞超微结构的影响[J]. 湖北农业科学, 2015, 54(16):3974-3977. doi:10.14088/j.cnki.issn0439-8114.2015.16.036.
L P H. Effects of Pb and Cd on chlorophyll content and cell ultrastructure of potato[J]. Hubei Agricultural Sciences, 2015,54(16):3974-3977.
[32] 黄佳璟, 林立金, 陈发波, 魏斩连, 温铿, 王铤, 刘磊, 廖明安, 任纬. 喷施褪黑素对萝卜生长及镉积累的影响[J]. 四川农业大学学报, 2017, 35(3):375-380. doi:10.16036/j.issn.1000-2650.2017.03.014.
Hang J J, Lin L J, Chen F B, Wei Z L, Wen K, Wang D, Liu L, Liao M A, Ren W. Effects of spraying melatonin on growth and cadmium accumulation of radish[J]. Journal of Sichuan Agricultural University, 2017, 35(3):375-380.
[33] 郭瞻宇, 张子杨, 蒋亚辉, Lahori A H, 张增强, 李荣华. 不同品种芥菜对镉胁迫的敏感性分布及抗氧化特征[J]. 农业环境科学学报, 2018, 37(12):2660-2668. doi:10.11654/jaes.2018-0202.
Go Z Y, Zhang Z Y, Jiang Y H, Lahori A H, Zhang Z Q, Li R H. Assessment of variation in sensitivity and antioxidant characteristics of different mustard cultivars under different Cd stress leaves[J]. Journal of Agro-environment Science, 2018,37(12):2660-2668.
[34] Salin M L.Toxic oxygen species and protective systems of the chloroplast[J].Physiologia Plantarum,1988,72(3):681-689. doi:10.1111/j.1399-3054.1988.tb09182.x.
[35] Zhang N, Zhao B, Zhang H J, Weeda S, Yang C, Yang Z C, Ren S X, Guo Y D. Melatonin promotes water-stress tolerance, lateral root formation, and seed germination in cucumber(Cucumis sativus L.)[J].Journal of Pineal Research, 2013, 54(1):15-23.doi:10.1111/j.1600-079X.2012.01015.x.
[36] Arnao M B, Hernández-Ruiz J. Chemical stress by different agents affects the melatonin content of barley roots[J].Journal of Pineal Research,2009,46(3):295-299.doi:10.1111/j.1600-079X.2008.00660.x.
[37] 王怀清, 刘志民. 褪黑素及其代谢物抗氧化作用研究进展[J]. 国际内分泌代谢杂志, 2009, 29(2):129-131. doi:10.3760/cma.j.issn.1673-4157.2009.02.018.
Wng H Q, Liu Z M. Progress on antioxidant properties of melatonin and its metabolites[J]. International Journal of Endocrinology and Metabolism, 2009, 29(2):129-131.
[38] Ma L Q, Rao G N. Chemical fractionation of cadmium, copper, nickel, and zinc in contaminated soils[J].Journal of Environmental Quality,1997,26(1):259-264.doi:10.2134/jeq1997.00472425002600010036x.
[39] 张磊,孟湘萍. 不同水分条件下镉在土壤中形态转化的动态过程[J]. 安徽农业科学, 2008, 36(17):7332-7334. doi:10.13989/j.cnki.0517-6611.2008.17.039.
Zang L, Meng X P. Dynamic process of the morphological transformation of cadmium in soil under different moisture conditions[J]. Journal of Anhui Agricultural Sciences, 2008, 36(17):7332-7334.
[40] Yu H Y, Liu C P, Zhu J S, Li F B, Deng D M, Wang Q, Liu C S. Cadmium availability in rice paddy fields from a mining area:the effects of soil properties highlighting Iron fractions and pH value[J].Environmental Pollution, 2016, 209:38-45. doi:10.1016/j.envpol.2015.11.021.
[41] 曹胜, 周卫军, 罗思颖, 周雨舟, 谭洁, 段群涛. 酸碱度调节剂对稻田土壤中有效态镉的影响研究[J]. 中国农学通报, 2017,33(30):97-102.
Co S, Zhou W J, Luo S Y, Zhou Y Z, Tan J, Duan Q T. Effect of pH adjustor on available cadmium in paddy soils[J]. Chinese Agricultural Science Bulletin, 2017, 33(30):97-102.
[42] Montiel-Rozas M M,Madejón E, Madejón P. Effect of heavy metals and organic matter on root exudates(low molecular weight organic acids)of herbaceous species:an assessment in sand and soil conditions under different levels of contamination[J]. Environmental Pollution, 2016, 216:273-281. doi:10.1016/j.envpol.2016.05.080.
[43] 王巧红, 董金霞, 张君, 杨万勤, 张健, 陈良华. Cd污染对3种类型土壤酶活性及Cd形态分布的影响[J]. 四川农业大学学报, 2017, 35(3):339-344. doi:10.16036/j.issn.1000-2650.2017.03.009.
Wng Q H, Dong J X, Zhang J, Yang W Q, Zhang J, Chen L H. Effects of Cd pollution on soil enzyme activities and Cd forms in three soil types[J]. Journal of Sichuan Agricultural University, 2017, 35(3):339-344.
[44] 郭碧林, 陈效民, 景峰, 张晓玲, 杨之江, 刘巍, 刘文心. 外源Cd胁迫对红壤性水稻土微生物量碳氮及酶活性的影响[J]. 农业环境科学学报, 2018, 37(9):1850-1855. doi:10.11654/jaes.2018-0344.
Go B L, Chen X M, Jing F, Zhang X L, Yang Z J, Liu W, Liu W X. Effects of exogenous cadmium on microbial biomass and enzyme activity in red paddy soil[J]. Journal of Agro-environment Science, 2018, 37(9):1850-1855.
[45] 罗虹, 刘鹏, 宋小敏. 重金属镉、铜、镍复合污染对土壤酶活性的影响[J]. 水土保持学报, 2006, 20(2):94-96, 121. doi:10.3321/j.issn:1009-2242.2006.02.023.
Lo H, Liu P, Song X M. Effect of compound pollution of Cd, Cu and Ni on soil enzyme activities[J]. Journal of Soil and Water Conservation, 2006, 20(2):94-96, 121.

Please choose a citation manager

Content to export