Screening and Analysis of Functional Substances in Chinese Chestnut Leaves Based on Metabolomics

doi:10.7668/hbnxb.20193807

Abstract

Abstract:

To provide a theoretical basis for the resource utilization of Chinese chestnut,the mature leaves of four chestnut cultivars were tested using ultra high performance liquid chromatography-tandem mass spectrometric,and functional secondary metabolites were screened by consulting relevant pharmacopoeias and searching the Chemical Book,Chemsrc,pharmacopeias and Web of Science database. The contents of 18 secondary metabolites we focused on in different cultivars were compared and analyzed. The study showed that a total of 673 kinds of secondary metabolites were detected in chestnut leaves,including flavonoids,phenolic acids,alkaloids,tannins,terpenoids,lignin and coumarin. Principal component analysis and cluster analysis showed that the difference of secondary metabolomics profile between Yanshanzaofeng and Yankui was little. The major secondary metabolites contributing to the classification were phloretin,didymin,salirepin,arbutin,cinnamic acid,gallocatechin,procyanidin B1 and so on. The differential metabolites could also be used as biomarkers to distinguish different chestnut varieties. A total of 202 functional secondary metabolites were selected,including 51 phenolic acids,24 alkaloids,83 flavonoids,21 terpenoids,14 lignins and coumarins,and 9 tannins. The contents of active substances were high in leaves such as protocatechuic acid,maslinic acid,ursolic acid,ellagic acid,chlorogenic acid,avicularin,kaempferol-7-O-glucoside,quercetin,spiraeoside,astragalin,betanin,tiliroside,ditymin and baimaside,which provided reference for the resource utilization of Chinese chestnut leaves.

Key words: Chinese chestnut, Leaf, Secondary metabolite, Functional substance

ZHANG Xinfang, ZHANG Shuhang, LI Ying, GUO Yan, WANG Guangpeng, WANG Yingjie. Screening and Analysis of Functional Substances in Chinese Chestnut Leaves Based on Metabolomics[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(S1): 245-252. doi: 10.7668/hbnxb.20193807.

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Figures/Tables 4

Fig.1 PCA analysis based on the secondary metabolome data of four Chinese chestnut cultivars R2X[1]and R2X[2]respectively mean explanation rate of principal component 1 and 2.

Fig.2 Loading plot for PCA analysis based on the secondary metabolome data of four Chinese chestnut cultivars pme1201.Phloretin;pmp000013.Eupatilin-7-O-glucoside;Li512111.Isorhamnetin-3-O-(6″-acetylglucoside);Zmhn002574.Rhamnazin 3-Glucosyl-arabinofuranoside;Lmmn001294.Koaburaside;mad2394.Sinapoyl-p-coumaroyltartaric acid;Hmtn001288.Methyl 2,4-dihydroxyphenylacetate;Zmpn006881.Tormentic acid;Lmsp013456.3β-Hydroxytaraxastan-28,20β-olide;Jmwn002117.2-(3,4-dihydroxyphenyl)ethanediol 1-O-β-D-glucopyranoside;Lmyp003599.Kaempferol-3-O-(6″-galloyl)glucoside;Lmpp003296.Delphinidin-3-O-glucuronide;Hmsn00259.Salirepin;Hmdn006685.3,4-Cycloopen-cycloaltin-4(29),25-diene-24(S)-hydroxy-3-carboxylic acid;MWSmce675.Arbutin;mws2213.Cinnamic acid;mws0049.Gallocatechin;pme3475.Butin;Hmlp003129.Matairesinol;pme0376.Naringenin (5,7,4'-Trihydroxyflavanone);Zmhn003046.4-O-(6'-O-Glucosylferuloyl)-3,4-dihydroxybenzyl alcohol;mws0836. Procyanidin B1;mws1179.Naringenin-7-O-glucoside;Lmpp003465.Myricetin-3-O-glucoside.

Tab.1 Types of functional secondary metabolites detected in Chinese chestnut leaves

物质二级分类 Class Ⅱ	物质数量 Compound number	功能分类 Functional classification	物质二级分类 Class Ⅱ	物质数量 Compound number	功能分类 Functional classification
生物碱Alkaloids	12	ABCFGHI	查耳酮Chalcones	7	ABCDFGHIJ
酚酸类Phenolic acids	51	ABCDEFGHIJ	二氢黄酮Flavanones	12	ABCDEFIJ
酚胺Phenolamine	2	FGI	二氢黄酮醇Flavanonols	2	ABDG
吲哚类生物碱Plumerane	7	BDHI	黄酮醇Flavonols	28	ABCDFG
吡咯类生物碱Pyrrole alkaloids	1	ABD	黄酮Flavones	23	ABCDIG
喹啉类生物碱Quinoline alkaloids	1	I	黄烷醇类Flavanols	7	ABCDFG
苯丙胺类生物碱Amphetamine alkaloids	1	GH	异黄酮Isoflavones	4	BCJ
倍半萜Sesquiterpenoids	2	BDFIJ	木脂素Lignans	5	ABDEI
倍萜Monoterpenoids	3	ABCDFG	香豆素Coumarins	9	ABDH
三萜Triterpene	13	ABCDGIJ	鞣质Tannin	6	ABEIJ
三萜皂苷Triterpene Saponin	3	ABDG	原花青素Proanthocyanidins	3	ABDG

Fig.3 Comparison of the content of important functional secondary metabolites in four Chinese chestnut cultivars Different lowercase letters indicate significant difference (P<0.05)(Fisher LSD test).

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