GhWRKY22 Isolation and Function Analysis in Cotton Resistance to Verticillium wilt

doi:10.7668/hbnxb.2018.06.022

Abstract

Abstract: The development of plant disease-resistant cultivars by disease-resistant gene mining becomes a most available measure. The bioinformation,virus induced gene silencing,qPCR and fungus inoculation were employed to analyze cotton WRKY resistance to Verticillium dahliae. Eight cotton orthologous WRKY genes corresponding to 8 Arabidopsis defense-related WRKYs was selected by phylogenetic tree analysis. And their expression was induced by V. dahliae infection,indicating that they participate in cotton defense to this fungus. Among these genes,six GhWRKYs showed up-regulation expression, and expression levels of GhWRKY70 and GhWRKY48 exhibited up-or down-regulation over time. Five cotton WRKY genes including showed two expression peaks at 24,72 h after pathogen inoculation. GhWRKY22 gene was chosen for further charactering its function in cotton resistance to V. dahliae infestation.GhWRKY22 expressed predominantly in stem,and its expression level was obviously increased upon pathogen infestation and responded positively to the defense signaling molecules such as salicylic acid and jasmonic acid hormones. Knockdown of GhWRKY22 gene increased susceptibility of plants to V. dahliae infection. Additionally,the expression level of defense marker genes,including PR1,PR3,PR4,PR5,PAL and PDF1.2,significantly decreased in GhWRKY22-silenced plants compared to the control plants,which suggesed that GhWRKY22 participates in cotton defense against Verticillium wilt through SA and JA signaling pathways. Taken together,these results showed that 8 GhWRKYs were identified and characterized in regulation of resistance to V. dahliae. Among them,GhWRKY22 was a positive regulation component in cotton defense,which could be regarded as a candidate gene to breed plant defense cultivars.

Key words: Gossypium hirsutum L., GhWRKY22, Disease-resistance, Verticillium dahliae, Gene silencing

CLC Number:

Q78
S435.62

LEI Yu, ZHANG Zhennan, HU Guang, LIU Jianfen, TANG Ye, ZHANG Ning, SI Huaijun, WU Jiahe. GhWRKY22 Isolation and Function Analysis in Cotton Resistance to Verticillium wilt[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.7668/hbnxb.2018.06.022.

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http://www.hbnxb.net/EN/Y2018/V33/I6/160

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