Isolation of Cotton Coronatine Insensitive Factor Gene (GhCOI1) and Its Function in Cotton Resistance Against Verticillium dahliae Infection

doi:10.7668/hbnxb.2017.01.005

Abstract

Abstract: Since Verticillium wilt takes a large loss in cotton production every year, cotton resistant cultivars developed by genetic engineering methods have been urgently demanded in cotton production. We isolated a cotton CORONATINE INSENSITIVE1(COI1)gene, named GhCOI1. The full-length cDNA of GhCOI1 gene was 2 213 bp, encoding a 600 amino acid peptide with a predicted molecular mass of 68 kDa and an isoelectric point (pI) of 7.06. GhCOI1 contains both typical F-box and LRR conserved domains. By RT-PCR and qPCR analyses, GhCOI1 gene was dominantly expressed in roots. And it was continuously induced overtime by Verticillium dahliae. The cotton plants with knock down of GhCOI1 gene were successfully developed by virus-induced gene silencing (VIGS) techniques. When VIGS-plants were challenged with V.dahliae, resistance of these plants significantly reduced according to analyses of rates of diseased plants and disease index compared to the control. All together, the results indicated that GhCOI1 was involved in cotton resistance to Verticillium wilt, which can be used as a candidate gene in the cotton resistant breeding.

Key words: Gossypium hirsutum L., GhCOI1 gene, Verticillium dahliae, Disease-resistance, Gene silencing

CLC Number:

Q78
S562.03

FAN Qiang, WANG Le, XIE Cailing, ZHANG Ning, SI Huaijun, WU Jiahe. Isolation of Cotton Coronatine Insensitive Factor Gene (GhCOI1) and Its Function in Cotton Resistance Against Verticillium dahliae Infection[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(1): 29-35. doi: 10.7668/hbnxb.2017.01.005.

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