Cloning and Activity Analysis of the Promoter of Photosynthetic Gene GhRCAα in Cotton

doi:10.7668/hbnxb.2017.01.004

Abstract

Abstract: In order to clone the promoter of rubisco activase gene(RCA)and understand its molecular regulation mechanism,we cloned and analyzed the cis-acting regulatory elements and activity of the 2 000 bp promoter sequence of GhRCAα from Baimian 1. The results showed that there were several important cis-acting elements, including the elements responsible for light, circadian, stress, phytohormone, and other basal elements in the upstream regulatory region of GhRCAα. Further analysis of the expression pattern of GhRCAα indicated that the expression of the GhRCAα was tissue specific, which accumulated at high levels in the photosynthesis organ of leaves, but at low levels in other detected tissues, which was consistent with the results that many light-responsive and organ-specific elements were present in the promoter region of GhRCAα. Transient expression analysis in tobacco leaf showed that 2 000 bp promoter sequence of GhRCAα was able to drive the GUS expression,suggesting that the cloned promoter fragment had the activity to drive the expression of target gene.These results indicated that the GhRCAα promoter was a tissue-specific promoter, which was expected to be used for the genetic transformation of plants, and thus to better control the specific expression of important genes.

Key words: Rubisco activase(RCA), Promoter, Cis-acting regulatory elements, Photosynthesis, Activity analysis, Cotton

CLC Number:

Q78
S562.03

CHAO Maoni, ZHANG Zhiyong, ZHANG Jinbao, WEN Qingyu, GUO Shulei, MA Liang, WANG Qinglian. Cloning and Activity Analysis of the Promoter of Photosynthetic Gene GhRCAα in Cotton[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(1): 21-28. doi: 10.7668/hbnxb.2017.01.004.

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