ACTA AGRICULTURAE BOREALI-SINICA ›› 2017, Vol. 32 ›› Issue (1): 36-40. doi: 10.7668/hbnxb.2017.01.006

Special Issue: Chinese cabbage

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Study on Tipburn Resistance QTL Mapping in Chinese Cabbage

JIN Xiuqing1, ZHANG Bin2, LI Mei2, WEN Fengying2, LIU Xiaohui2, WANG Chaonan2, HUANG Zhiyin2   

  1. 1. College of Life Sciences, Tianjin Normal University, Tianjin 300387, China;
    2. Tianjin Kernel Vegetable Research Institute, Tianjin 300384, China
  • Received:2016-09-19 Published:2017-02-28

Abstract: Different tipburn resistance materials of Chinese cabbage were used as materials to study the inheritance of resistance gene to tipburn and to conduct QTL mapping so as to provide a theoretical basis for the molecular assistant selection (MAS) breeding and resistance mechanism. This research adopted significantly different resistance tipburn line high inbred Qingmaye type Black 227 (resistant lines) and high inbred Baotou type B120 (susceptible lines) as materials to obtain DH group by microspore culture F1 generation. We classified degrees of the disease and combined the molecular genetic map of Chinese cabbage. According to disease severity in the field of DH group, QTL analysis on tipnurn resistance genes were conducted by MapQTL 5.0 software. Results showed that the inheritance of the resistance gene to Chinese cabbage tipburn in tested materials fit to the inheritance law of quantitative trait.This study detected two InDel markers BrID10343 and BrID10349 which link closelyed with tipburn resistance gene of Chinese cabbage. Two markers were on the Chr.7,in which the genetic distance of 1.031 cM and genetic contribution rates were above 40%.InDel markers BrID10343 and BrID10349 linked closelyed with tipburn resistance gene of Chinese cabbage. The results would be of great benefit to fine mapping for the major QTL of tipburn resistance gene, also the results will lay a good foundation for Chinese cabbage MAS resistance breeding.

Key words: Chinese cabbage, Tipburn, QTL mapping

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Cite this article

JIN Xiuqing, ZHANG Bin, LI Mei, WEN Fengying, LIU Xiaohui, WANG Chaonan, HUANG Zhiyin. Study on Tipburn Resistance QTL Mapping in Chinese Cabbage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(1): 36-40. doi: 10.7668/hbnxb.2017.01.006.

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