Abstract: In order to determine the occurrence and species of root maggot pests in cultivated Chuanxiong fields in Anguo City, Hebei Province, the larvae of root maggot were collected and reared in simulated field habitats. The species of root maggots of Chuanxiong in Anguo City were identified by using traditional morphological classification technology and modern molecular biological classification technology. The damage characteristics and degree of root maggots of Chuanxiong in Anguo City were systematically investigated by five-point sampling method. The results showed that the damage rate of root maggots was 4%-82%, the death rate of seedlings was 22%, and the average number of insects per plant was 37.76. The root maggots of Chuanxiong mainly damaged the root and stem of Chuanxiong through larvae, resulting in yellowing of the upper leaves of Chuanxiong, and the whole plant died when the roots were eaten completely. By comparing the traditional morphological characteristics of the adults, it was found that the morphological characteristics of the root maggot of Chuanxiong in Anguo were basically the same as those of the Bradysia odoriphaga, and the results of molecular marker identification showed that the sequence of mtDNA-COI gene of the root maggot of Chuanxiong in Anguo was 99% consistent with that of the B. odoriphaga. Root maggots were B. odoriphaga larvae. The results of this study showed that the root maggot species harmed and seriously affected the yield and quality of Chuanxiong in Anguo City of Hebei Province in recent years were B. odoriphaga, and the damage characteristics and degree of the root maggot of Chuanxiong were clarified, which would provide a scientific basis for the green control of the root maggot of Chuanxiong in the future.

Key words: Ligusticum chuanxiong Hort, Root maggot, Molecular marker, Species identification, Bradysia odoriphaga

CLC Number: 

• S436.3