Study on Differential Expression of OAZ1 and OAZ2 Gene in Various Tissues of the Sichuan White Goose

doi:10.7668/hbnxb.2014.01.010

Abstract

Abstract: The objective of this work was to elucidate the expression profiling of OAZ1 and OAZ2 in various tissues of Sichuan white geese. The relative abundance of OAZ1 and OAZ2 mRNA in thirteen tissues of Sichuan white geese were determined by quantitative real-time PCR. The ratio between the amounts of OAZ1 and OAZ2 gene expression was assessed. OAZ1 mRNA was most highly expression in brain and spleen,8. 74-fold and 8. 45-fold,respectively,compared with that in heart(P <0. 05). The amount of OAZ1 gene expression in breast,thigh muscle and ovarian tissues was lower,0. 15-fold,0. 42-fold and 0. 46-fold,respectively,compared with that in heart. The level of OAZ2 mRNA expression in retina was higher 6. 79-fold compared with that in heart(P <0. 05). The relative abundance of OAZ2 in liver,breast and thigh muscle was lower,0. 47-fold,0. 08-fold and 0. 19-fold,respectively,compared with that in heart(P <0. 05). OAZ2 mRNA was more abundant than that of OAZ1 in breast and thigh muscle. OAZ1 mRNA was more abundant than that of OAZ2 in other determined tissues in the experiment. The highest OAZ1 / OAZ2 ratio was found in ovary and cerebellum(26. 62 and 17. 72,respectively),whereas the lowest one was in breast and thigh muscle(0. 33 and 0. 75,respectively). OAZ1 and OAZ2 mRNA are ubiquitously and tissue-specificity expressed. The OAZ1 / OAZ2 ratio is dependent on the different tissue of Sichuan white goose. OAZ1 and OAZ2 may exert differently regulatory function in various tissues.

Key words: Sichuan white goose, OAZI, OAZ2, Gene expression, OAZ1/OAZ2

CLC Number:

YI Zhi-xin, JIANG Dong-mei, KANG Bo. Study on Differential Expression of OAZ1 and OAZ2 Gene in Various Tissues of the Sichuan White Goose[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(1): 50-54. doi: 10.7668/hbnxb.2014.01.010.

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