Study on the Molecular Variability of Immunoglobulin IgG/IgM from Goslings Infected Goose Parvovirus

doi:10.7668/hbnxb.2015.01.008

Abstract

Abstract: To conduct isolating and purifying of immunoglobulin IgG/IgM from goslings infected Goose parvovirus YZ strain(GPV-YZ) and make analysis of protein structure and function by biochemical and molecular biology, and research the characteristics of Ig and the physicochemical properties and pathogenic mechanism of IgG/IgM protein.Immunoglobulin(Ig) was isolated and purified from goslings infected Goose parvovirus(RD)and control group(CK)by ammonium sulphate fractionation chromatographies on Sephadex G-100 and Utro-gel ACA22.The results of SDS-PAGE and biochemical analysis showed that there were deleted 6 main protein(128, 114, 69, 59, 55, 48 kDa) band of the primary sedimentation Ig from serum of goslings infected Goose parvovirus in non reductive conditions, but under reductive conditions, deleted 7 main protein(139, 128, 119, 113, 97, 94, 61 kDa) band and appeared three new main protein(64, 65, 36 kDa) band of the primary sedimentation Ig from serum of goslings infected Goose parvovirus.There were increased respectively 10, 7 times or 1.5, 2 times of protein bands of IgM and IgG from goslings infected Goose parvovirus and control group, and indicated that the high variability macromolecular IgM/G monomers caused an abundant polymorphism in shape configuration, and the IgM/G subunit change was an important marker of the resistance potential in goslings.There were deleted 150 kDa band and heavy chain (64 kDa) of the purified IgG from serum of goslings infected Goose parvovirus in non reductive conditions, but under reductive conditions, the IgG showed heavy chain(60~70 kDa) characteristic protein band in goslings infected Goose parvovirus and control group.The 62 kDa specific heavy chain bands only appeared in the IgM/G of control group, and deleted the 91 kDa, IgG(ΔFc)(43 kDa)and light chain (25 kDa) protein band from serum of goslings infected Goose parvovirus.Those indicated that there existed the interaction between GPV infection and Ig.The IgG is associated with GPV infection, and 62 kDa heavy chain gene might be the susceptible gene of GPV.The results also indicated that the Ig content of goslings infected Goose parvovirus was only 37 percent of control group, and IgG content was 2.56 times higher than that of IgM in serum of goslings infected Goose parvovirus. The specific activity of IgM/G of goslings infected Goose parvovirus was significantly higher than the control group. Those indicated that the expression of immunoglobulin gene were reduced by goslings infected Goose parvovirus, and stimulated goslings infected Goose parvovirus.The specific activity of IgM/G of goslings infected Goose parvovirus was significantly higher than the control group.Those indicated that the expression of immunoglobulin gene were reduced by goslings infected Goose parvovirus, and stimulated goslings infected Goose parvovirus.The stability test showed that the IgG was pH stability better than IgM in goslings infected Goose parvovirus, but the IgM was better thermal stability than IgG in goslings infected Goose parvovirus and IgM/G were more sensitive to alkali and temperature in goslings infected Goose parvovirus.Those indicated that with temperature, pH different immunoglobulin might also occur many reactions, and the alkali and temperature sensitivity of Ig increased in goslings serum infected Goose parvovirus.

Key words: Goslings, Goose parvovirus, Immunoglobulin M/G, SDS-PAGE, Gel chromatography

CLC Number:

S853.5
Q78

ZHU Xin-chan, SHAO Yan-hong, ZHU Feng-wei, YANG Li-jin. Study on the Molecular Variability of Immunoglobulin IgG/IgM from Goslings Infected Goose Parvovirus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(1): 42-53. doi: 10.7668/hbnxb.2015.01.008.

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