The Genotyes and Inheritance of High-Molecular-Weight Glutenin Subunits(HMW-GS) in Transgenic Wheat by SDS-PAGE

doi:10.3321/j.issn:1000-7091.2005.02.001

Abstract

Abstract: The genotypes and inheritance of High-Molecular-Weight Glutenin Subunits (HMW-GS) in T₂～T₅ generations transgenic wheat plants were conduced by SDS-PAGE. The results showed that the exogenous 1Dx5 and 1Dy10 genes expressed in some plants in T₂ population, and in some plants caused some endogenous genes to be null and a new subunit to appear. Many genotypes such as 2+5+7+10+12, 2+5+7+8^*+10+12, 5+7+10, 2+7+10+12, therefore, were picked out. The lines with 2+5+10+12 continue to separate from T₂ to T₅ in offspring populations of different plants, different spikes form one plant or different grains form one spike. So we get some stably expressed novel wheat lines with 2+5+10+12, 2+10+12, 2+5+12, which do not exist in nature were obtained.

Key words: Transgenic, Wheat, HMW-GS, SDS-PAGE, Separation

CLC Number:

S512.1

YANG Feng-ping, LIANG Rong-qi, CHEN Xu-qing, HAN Li-xin, ZHANG Xiao-dong. The Genotyes and Inheritance of High-Molecular-Weight Glutenin Subunits(HMW-GS) in Transgenic Wheat by SDS-PAGE[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2005, 20(2): 1-4. doi: 10.3321/j.issn:1000-7091.2005.02.001.

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