Effects of CPPU on Protein Response of Wheat Seedlings Leaves and Different Growth Stage under Drought Stress

doi:10.3969/j.issn.1000-7091.2012.02.034

Abstract

Abstract: This experimentation focused on the effects of CPPU on wheat seedlings proteins and physiology-biochemistry indexes under drought stress to explore the molecular mechanisms of CPPU treatment on improving?drought resistance of wheat by foliage spraying with CPPU(N-(2-chloro-4-pyridyl)-N-phenylurea)． With Henong?822 as materials，SDS-PAGE was used to study changes of proteomics in seedlings leaves，and routine methods were?used to test changes of peroxidase (POD) activity，the contents of soluble sugar and protein，proline and malondialdedyde (MDA) contents． The result of SDS-PAGE showed that seven specific electrophoresis bands were obtained?after CPPU treatmant compared with the control under drought stress，their molecular weight was 125，80，55，40, 35，33 and 25 kDa respectively，but one of 30 kDa was disappeared． In compared with the drought control，40 kDa?specific band was found in protein bands of leaves with CPPU treatment，but 30 kDa strip was disappeared． The protein contents of 70 kDa and about 20 kDa were higher than two contrast groups，while the protein contents of 32 kDa?and about 25 kDa were decreased． After spraying 100 mg CPPU，POD activity was increased by 37． 06%，the contents of soluble protein and sugar were improved by 63． 50% and 87． 51%，proline content was improved by 109．?57%，but MDA content was decreased by 46． 06% in wheat seedlings under drought stress． It illustrated spraying?CPPU on leaves could strengthen adaptability of wheat under drought stress． In compared with the drought control，one specific protein band was found in seedlings leaves of treatment with CPPU，one was disappeared and the protein contents of 70 kDa and about 25 kDa were higher than two contrast groups，while the protein contents of 32 kDa?and about 25 kDa were decreased． 100 mg of CPPU could improve the contents of soluble sugar，protein and proline?as well as POD activity significantly，but decrease MDA content in wheat seedlings under drought stress． It illustrated spraying CPPU on leaves could strengthen adaptability of seedlings under drought stress．

Key words: CPPU, Wheat, Drought stress, SDS-PAGE

CLC Number:

S512.01

ZHAO Fang, YAO Zhan-jun, SU Ji-hua. Effects of CPPU on Protein Response of Wheat Seedlings Leaves and Different Growth Stage under Drought Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(2): 180-184. doi: 10.3969/j.issn.1000-7091.2012.02.034.

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