ACTA AGRICULTURAE BOREALI-SINICA ›› 2008, Vol. 23 ›› Issue (5): 85-88. doi: 10.7668/hbnxb.2008.05.018

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Protein Pattern of Kidney Bean Seed and Changes during Kidney Bean Seed Germination

DANG Gen-you, FENG Bai-li, GAO Xiao-li, GAO Jin-feng, GAO Dong-li, CHAI Yan   

  1. College of Agronomy, Northwest Agricultural and Forestry University, Yangling 712100, China
  • Received:2008-05-10 Published:2008-10-28

Abstract: Four kidney bean cultivars with significant difference were used to research the changes of the protein patterns of the storage proteins in the cotyledon and embryo of three protein kinds(water soluble proteins,salt soluble proteins and total proteins)during kidney bean seed germination by SDS-PAGE,the results showed that:T here were plentiful storage proteins in subunits in the cotyledon and embryo,thereinto,the significant difference of the storage proteins in subunits was found in the cotyledon and embryo of one cultivar,there were less diversities and plentiful storage protein patterns among water soluble proteins,salt soluble proteins and total proteins patterns;There were differences in protein patterns among the cultivars.During kidney bean seed germinat ion the storage prote in subunits of macromolecule were mobilized more quickly than lowermolecule,the storage prote in subunits of the cotyledon were mobilized more slowly than embryo,there were significant differences in protein patterns of the cotyledon and embryo among the cultivars,the cultivars of the hige seed vigor(Y05,Y06)were mobilized more rapidly than the varieties of the low seed vigor(Y09,Y1+1),and there were not diversities between the variet ies of the high seed protein content(Y1+1,Y05)and the variet ies of the low seed protein content(Y06,Y09).The results provided the theoretical basis for the ident ification of crop varieties,the improvement of crop varieties,quality culture and the mechanism of germination among the kidney beans.

Key words: Kidney bean, Storage proteins, Cotyledon and embryo, Seeds germination, SDS-PAGE, Prote in subunit

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Cite this article

DANG Gen-you, FENG Bai-li, GAO Xiao-li, GAO Jin-feng, GAO Dong-li, CHAI Yan. Protein Pattern of Kidney Bean Seed and Changes during Kidney Bean Seed Germination[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(5): 85-88. doi: 10.7668/hbnxb.2008.05.018.

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