Molecular Improvement of Rice Blast Resistance in a Rice Maintainer Line Ruanhua B Based on MAS

doi:10.7668/hbnxb.20193407

Abstract

Abstract:

In order to improve blast resistance of the maintainer line Ruanhua B,to carry rice blast resistance genes Pi46 and Pi2 high-quality Indica H281 as the donor parent,Ruanhua B as recurrent parent,using marker-assisted selection(MAS)technology combined with pedigree breeding method,polymerization of two foreign genes with improved maintenance line Ruanhua B resistance,Ruanhua B was carried out on the characteristics of stable strain identification of resistance to rice blast,rice quality analysis,etc.Two BC₁F₆ populations,two BC₂F₅ populations and two BC₃F₄ populations with two homozygous target genes were obtained by backcrossing,multi-generation self-crossing and molecular marker detection.Field naturally induced identification showed that the improved lines of different backcrossing generations were resistant to rice blast.The sterility of backcross generation to sterile lines ranged from 52.7% to 100.0%.Agronomic traits and rice quality analysis showed that the improved lines basically conserved the main agronomic characters and rice quality characteristics of Ruanhua B.The results of SNP gene chip analysis showed that the background response rate of BC₁F₆was 74.42%—77.77%,that of BC₂F₅ was 86.42%—87.75%,and that of BC₃F₄ was 92.27%—92.59%.Multiple resistance genes can be effectively polymerized by continuous backcross,self-cross and marker-assisted selection techniques to obtain a new maintainer line resistant to rice blast,and achieve rapid molecular improvement of maintainer line Ruanhua B.

Key words: Rice, Maintainer line, Rice blast, Molecular marker-assisted selection, Rice quality, Genetic background

ZHAO Zhe, WANG Yujiang, LIANG Jiecai, LIU Yongzhu, ZHOU Jiyong, CHEN Xionghui, LIANG Keqin, XIAO Wuming. Molecular Improvement of Rice Blast Resistance in a Rice Maintainer Line Ruanhua B Based on MAS[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(1): 144-153. doi: 10.7668/hbnxb.20193407.

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References 64

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引物名称 Marker	类型 Marker type	染色体 Chromosome	正向引物序列(5'—3') Forward primer sequence(5'—3')	反向引物序列(5'—3') Reverse primer sequence(5'—3')	退火温度/℃ Annealing temperature	分离方法 Separation
RM224	SSR	11	ATCGATCGATCTTCACGAGG	TGCTATAAAAGGCATTCGGG	56	8% PAGE
Pi2-23	InDel	6	CGGTAAGAGTAACACCAAGC	GACGTGCGAGTTGTGACAGCT	56	8% PAGE

引物名称 Marker	类型 Marker type	染色体 Chromosome	正向引物序列(5'—3') Forward primer sequence(5'—3')	反向引物序列(5'—3') Reverse primer sequence(5'—3')	退火温度/℃ Annealing temperature	分离方法 Separation
RM224	SSR	11	ATCGATCGATCTTCACGAGG	TGCTATAAAAGGCATTCGGG	56	8% PAGE
Pi2-23	InDel	6	CGGTAAGAGTAACACCAAGC	GACGTGCGAGTTGTGACAGCT	56	8% PAGE

株系 Lines	受体背景回复率 RP genome	理论背景回复率 Theoretical recovery rate	供体背景回复率 DP genome	杂合度 Residual heterozygosity
L1	77.77	75.00	19.04	3.19
L2	74.42	75.00	21.91	3.67
L3	87.75	87.50	7.78	4.47
L4	86.42	87.50	8.83	4.75
L5	92.59	93.75	2.32	5.09
L6	92.27	93.75	2.47	5.26
平均值Mean	84.77	85.42	10.39	4.41

株系 Lines	受体背景回复率 RP genome	理论背景回复率 Theoretical recovery rate	供体背景回复率 DP genome	杂合度 Residual heterozygosity
L1	77.77	75.00	19.04	3.19
L2	74.42	75.00	21.91	3.67
L3	87.75	87.50	7.78	4.47
L4	86.42	87.50	8.83	4.75
L5	92.59	93.75	2.32	5.09
L6	92.27	93.75	2.47	5.26
平均值Mean	84.77	85.42	10.39	4.41

株系 Lines	叶瘟等级 Rating of infected leaves	穗瘟等级 Rating of infected panicles	抗病等级 Rating of resistance level
软华B	8	9	高感
Ruanhua B
H281	3	3	抗
L1	3	5	抗
L2	3	5	抗
L3	3	5	抗
L4	3	3	抗
L5	3	3	抗
L6	3	3	抗

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