Effects of Neurospora crassa NC-3 Strain on Degradation of Rice Straw

doi:10.7668/hbnxb.20193067

Abstract

Abstract:

To clarify the application potential of Neurospora crassa NC-3 strain on rice straw returning,a combination of indoor simulation and pot experiment were conducted to investigate the effects of NC-3 strain on degradation,cellulose,hemicellulosic,lignin,total phenolic acid content of rice straw,germination rate and seedling rate of rapeseed.The results showed that NC-3 strain could rapidly colonize on sterilized rice straw(full of mycelia and spores at 72 h).Compared with the sterile water,the degradation rate of rice straw in NC-3 strain was increased by 2.3,7.3 and 3.2 percentage points at 7,14 and 21 days,respectively.The contents of cellulose,lignin and total phenolic acid decreased with NC-3 strain by 2.0,10.7,10.4 percentage points,0.7,0.9,1.3 percentage points and 7.6%,6.9%,6.4%,respectively.The degradation effect of NC-3 strain on cellulose,lignin and total phenolic acid of rice straw mainly occurred in the first two weeks(0—14 days)of straw,and the degradation effect on hemicellulose gradually increased after 14 days of culture.Addition of rice straw significantly reduced the germination rate and seedling rate of rapeseed,and with the increase of straw dosage,the inhibition effect was enhanced,but NC-3 strain could significantly increase the germination rate and seedling rate of rapeseed(3.3 and 9.7 percentage points higher than sterile water in petri dish test,respectively).In summary,NC-3 strain could effectively accelerate the degradation of rice straw and the transformation of phenolic acids,and effectively improve the germination rate and seedling formation rate of rapeseed.

Key words: Neurospora crassa, NC-3 strain, Rice straw, Degradation, Rapeseed seedling

LI Yinshui, YU Changbing, DAI Zhigang, GU Chiming, LI Yue, LIAO Xing, XIE Lihua, HU Xiaojia, QIN Lu. Effects of Neurospora crassa NC-3 Strain on Degradation of Rice Straw[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(1): 154-161. doi: 10.7668/hbnxb.20193067.

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Figures/Tables 8

Fig.1 Colonization of Neurospora crassa (NC-3)on rice straw A、C.CK 24,72 h;B、D.NC-3 24,72 h。Bars=1.0 mm。

Fig.2 Effect of Neurospora crassa (NC-3) on degradation rate of rice straw Different letters are significantly different at 0.05 level.The same as Fig.3—8.

Fig.3 Effect of Neurospora crassa (NC-3) on cellulose content in rice straw

Fig.4 Effect of Neurospora crassa (NC-3) on hemicellulose content in rice straw

Fig.5 Effect of Neurospora crassa (NC-3) on lignin content in rice straw

Fig.6 Effect of Neurospora crassa (NC-3) on total phenolic acid content in rice straw

Fig.7 Effect of Neurospora crassa (NC-3) on germination rate and seedling rate of rapeseed

Fig.8 Effect of Neurospora crassa (NC-3) on germination rate and seeding rate of rapeseed

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