The Screening of a Carbofuran-degrading Bacteria Strain Achromobacter sp. CF-14 and Its Degradation Conditions Research

doi:10.7668/hbnxb.2011.S1.046

Abstract

Abstract: Carbofuran is a kind of high residue,high toxicity,difficult degradation organic pesticide,which is widely used in China and often caused human and animal poisoning and environmental pollution.We isolate one strain carbofuran-degrading bacterium from the soil of perennial using carbofuran by enrichment separation method.It was identified as Achromobacter sp according to its biochemical characteristics,morphology and 16 S rDNA molecular biology appraisal.The results of the degrading tests showed that the preliminary culture conditions were temperature 30℃,pH 7.0 and inoculation quantity 10%.It degraded carbofuran in the rate of 95% for 3 days.

Key words: Carbofuran, Degradation, Achromobacter

CLC Number:

S482

JING Xin-tang, LI Qin-fan, ZHANG Li-hui, KONG Xiang-ya. The Screening of a Carbofuran-degrading Bacteria Strain Achromobacter sp. CF-14 and Its Degradation Conditions Research[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(S1): 229-232. doi: 10.7668/hbnxb.2011.S1.046.

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http://www.hbnxb.net/EN/Y2011/V26/IS1/229

References

[1] Chaudhry G R，Ali H D．Bacterial metabolism of carbofuran[J]．Applied and Environmental Microbiology，1988，54(6):1414-1419．
[2] Bano N，Musarrat J．Characterization of a novel carbofuran degrading Pseudomonas sp．with collateral biocontrol and plant growth promoting potential[J]．FEMS Microbiology Letters，2004，231(1):13-17．
[3] Ogram A V，Duan Y，Trabue S L，et al．Carbofuran degradation mediated by three related plasmid systems[J]．FEMS Microbiology Ecology，2000，32(3):197-203．
[4] Kim I S，Ryu J Y，Hur H G，et al．Sphingmonas sp．Strain SB5 degrades carbofuran to a new metabolite by hydrolysis at the furanyl ring[J]．Journal of Agricultural and Food Chemistry，2004，52(8):2309-2314．
[5] Zhi L，Qing H，Xu J H，et al．Construction of a genetically engineered microorganism for degrading organophosphate and carbamate pesticides[J]．International Biodeterioration＆ Biodegradation，2006(58):65-69．
[6] 余磊，秦宝福，冯贵颖，等．一株克百威降解菌的分离及其降解特性研究[J]．西北农业学报，2009，18 (3):277-281．
[7] Holt J G，Krieg N G，Sneath P H A，et al．Bergey's manual of determinative bacteriology[M]．Willims and Wilkings Baltimore，1994．
[8] 东秀珠，蔡妙英．常见细菌系统鉴定手册[M]．北京:科学出版社，2001．
[9] 李玉梅，王根林，于洪久，等．呋喃丹降解菌的分离与特性研究[J]．大豆科学，2009，28(2):281-284．
[10] 刘宪华，宋文华，戴树桂．呋喃丹降解菌AEBL3 的筛选及特性研究[J]．上海环境科学，2003，22(11):743－ 745．
[11] 武俊，徐剑宏，洪青，等．一株呋喃丹降解菌(CDS－ l) 的分离和性状研究[J]．环境科学学报，2004，24 (2):338-342．
[12] Suett D L．Accelerated degradation of carbofuran in previously treated field soils in the United Kingdom[J]．Crop Protection，1986，5(3):165-169．
[13] Venkateswarlu K，Sethunathan N．Degradation of carbofuran by Azospirillum lipoferum and Streptomyces spp．isolated from flooded alluvial soil[J]．Bulletin of Environmental Contamination and Toxicology，1984，33(1):556-560．
[14] Ryeol P M，Lee S，Han T H，et al．A new intermediate in the degradation of carbofuran by Sphingomonas sp．strain SB5[J]．Journal of Microbiology and Biotechnology，2006，16:1306-1310．
[15] 武俊，洪青，陈一楠，等．GC/MS 和GC/FTIR 分析细菌降解呋喃丹的产物[J]．光谱学与光谱分析，2006，26(9):1716-1719.

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