SSR Analysis on 36 Potato Varieties

doi:10.3969/j.issn.1000-7091.2012.01.017

Abstract

Abstract: To know the genetic differences in DNA level among 36 potato varieties, the polymorphism among the tested plants were analyzed based on SSR molecular marker. 10 primer combinations, which suited the genome DNAs of 36 potato varieties, were selected from the 90 primer combinations. 301 bands of SSR markers were amplified, the percentage of polymorphism bands reached 71. 1%. The differences in SSR fingerprints amplified with primers STACCA3 and SSR111 were significant. It can be used as molecular evidence to distinguish 36 potato varieties. The genetic distance(GD) of 36 tested plants ranged from 0. 33 to 0. 85, and the average of genetic distance was 0. 54. There were 20 varieties, GD of which was greater than the average GD, accounting for 55. 56% of the varieties tested. Using the GD value of 0. 57 as threshold, 36 tested plants could be clustered into 7 groups, the genetic relationships among tested varieties were revealed at DNA molecular level.

Key words: Potato, Varieties, SSR analysis, Genetic difference

CLC Number:

S533

ZHANG Zi-qiang, YU Xiao-xia, JU Tian-hua, MA Yan-hong, YU Zhuo, MENG Mei-lian, LI Chang-qing. SSR Analysis on 36 Potato Varieties[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(1): 93-97. doi: 10.3969/j.issn.1000-7091.2012.01.017.

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