Identification and Analysis of miRNAs in Early-maturing Cotton by High-Throughput Sequencing

doi:10.7668/hbnxb.20190698

Abstract

Abstract: The amount of miRNA identified in cotton is still limited and the role of miRNAs in early-maturing cotton remains to be proved and revealed. In order to enrich the amount of miRNA in cotton and reveal the important roles of miRNA in the development of early-maturing cotton, two small RNA libraries were constructed using Shizao 1, a early-maturing cotton variety and Jimian 958, a medium cotton variety by high-throughput sequencing technology. As a result, a total of 131 miRNAs were identified, including 64 known miRNAs belonging to 41 miRNA family and 67 novel miRNAs; 54 of the 67 novel miRNAs belonged to known miRNA family and 13 novel miRNAs belonged to novel miRNA family. The expression of miRNAs were compared in the two cotton varieties, it found that 39 miRNAs showed obviously different expression(>2 fold) between the two varieties, which accounted for 29.8% and focused on low- and mid-expressed miRNAs. In the top 15 highly expressed miRNAs, only ghr_21(miR398 family) showed obviously different expression(3.4 fold). The expression of miRNAs belonging to the same family were compared and found different expression pattern in the two varieties. Above results revealed that miRNAs had important roles of earliness in the development of early-maturing cotton, which provide a basis for further study on the role of miRNA in the regulation of cotton prematurity.

Key words: Cotton, miRNA, High-throughput sequencing, Earliness

CLC Number:

S562.03

DONG Zhanghui, ZHU Qingzhu, ZHAO Lifen, SUI Shuxiang, LI Zengshu, ZHANG Yanli, WANG Hu, GUO Yongzhao, YAO Zeyang, ZHAO Guozhong. Identification and Analysis of miRNAs in Early-maturing Cotton by High-Throughput Sequencing[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(S1): 15-21. doi: 10.7668/hbnxb.20190698.

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