Effects of Different Mycorrhizal Fungi on Growth and Root System of Plug Seedlings of Dendrobium officinale

doi:10.7668/hbnxb.20192698

Abstract

Abstract:

In order to explore the symbiotic relationship between mycorrhizal fungi and Dendrobium officinale plug seedlings, the aim was to screen out the best and excellent symbiotic fungal strains that affect the growth of the plug seedlings.Different Orchidaceae mycorrhizal fungi and plug seedlings of Dendrobium officinale were used as experimental materials to study the symbiotic relationship between mycorrhizal fungi and plug seedlings, so as to screen out the best symbiotic fungi strains that affect the growth of plug seedlings of Dendrobium officinale.The results showed that:The average fresh weight growth rates of PS1, PS2 and PS3 were 15.7, 7.4 and 19.7 percentage higher than those of the control group, and the differences were extremely significant (P<0.01);The root growth rate of the plug seedlings PS1, PS2, PS3 and PS5 was 44.4, 43.6, 30.2 and 33.4 percentage higher than that of the control group, the difference reached a very significant level (P<0.01).The D4 strain which belonged to the same genus of Epulorhiza sp., showed inhibitory effect during the entire culture process, and the promotion effect of the D5 strain was more obvious in the later stage than in the early stage.It can also be seen from the change of chlorophyll content that other strains except D4 could significantly increase the formation of chlorophyll and promote the growth of plug seedlings.The best excellent strain selected in this research experiment was D1 strain (Epulorhiza sp.), D2 strain (unknown) and D3 strain (Rhizoctonia sp.) significantly promoted the growth of Dendrobium officinale plug seedlings. This would soon be applied to the subsequent transplantation and domestication of tissue culture seedlings of Dendrobium officinale, and would also provide theoretical and practical reference for the efficient in vitro culture and propagation of embryos of other orchidaceous medicinal plants.

Key words: Dendrobium officinale, Orchid mycorrhizal fungi, Plug seedlings

LI Jinghong, ZHANG Lihua, ZHONG Jing, DUAN Liansheng. Effects of Different Mycorrhizal Fungi on Growth and Root System of Plug Seedlings of Dendrobium officinale[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(S1): 172-177. doi: 10.7668/hbnxb.20192698.

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References 22

[1]	Selosse M A, Boullard B, Richardson D. Noël Bernard (1874-1911):Orchids to symbiosis in a dozen years, one century ago[J]. Symbiosis, 2011, 54(2):61-68. doi:10.1007/s13199-011-0131-5. doi: 10.1007/s13199-011-0131-5 URL
[2]	Dmitriev G, Malysheva E, Malysheva V, Tiunov A, Kovalenko A, Voronina E Y. Biodiversity of mycobionts and fungal root endophytes and trophic strategies of Goodyera repens (L.) R.BR.(Orchidaceae)[C]. Moscow:4th Congress of Russian Mycologists, 2017.
[3]	杨建文, 凌鸿, 张盈, 曾旭, 郭顺星. 内生真菌对兰科药用植物种子萌发作用研究进展[J]. 菌物学报, 2018, 37(1):22-34. doi:10.13346/j.mycosystema.170198. doi: 10.13346/j.mycosystema.170198
	Yang J W, Ling H, Zhang Y, Zeng X, Guo S X. Effects of endophytic fungi on seed germination of medicinal plants of Orchidaceae:a review[J]. Mycosystema, 2018, 37(1):22-34.
[4]	曾旭, 杨建文, 凌鸿, 张盈, 郭顺星. 石斛小菇促进天麻种子萌发的转录组研究[J]. 菌物学报, 2018, 37(1):52-63. doi:10.13346/j.mycosystema.170197. doi: 10.13346/j.mycosystema.170197
	Zeng X, Yang J W, Ling H, Zhang Y, Guo S X. RNA-Seq analysis of Gastrodia elata seed germination infected with Mycena dendrobii[J]. Mycosystema, 2018, 37(1):52-63.
[5]	李景蕻, 张丽华. 药用兰科植物组培苗菌根化技术在种苗生产中的应用[J]. 种子, 2015, 34(8):133-134. doi:10.16590/j.cnki.1001-4705.2015.08.133. doi: 10.16590/j.cnki.1001-4705.2015.08.133
	Li J H, Zhang L H. Application of tissue culture and mycorrhizal technology of medicinal orchid plants for seedling production[J]. Seed, 2015, 34(8):133-134.
[6]	陈娟, 孟志霞, 邢咏梅, 郭顺星. 5种兰科药用植物可培养内生真菌的鉴定及多样性分析[J]. 中国药学杂志, 2017, 52(4):267-271. doi:10.11669/cpj.2017.04.004. doi: 10.11669/cpj.2017.04.004
	Chen J, Meng Z X, Xing Y M, Guo S X. Isolation and identification of endophytic fungi from five medicinal plants species of Orchidaceae[J]. Chinese Pharmaceutical Journal, 2017, 52(4):267-271.
[7]	阎波, 陈娟, 郭顺星. 植物菌根共生中的激素调控作用研究进展[J]. 植物生理学报, 2017, 53(6):916-924. doi:10.13592/j.cnki.ppj.2016.0450. doi: 10.13592/j.cnki.ppj.2016.0450
	Yan B, Chen J, Guo S X. Advances in regulating effects of hormones in mycorrhizal symbiosis[J]. Plant Physiology Journal, 2017, 53(6):916-924.
[8]	王珊珊, 刘佳萌, 孙晶, 贾宁, 范蓓, 戴小枫. 药用石斛内生菌的研究进展[J]. 中国食物与营养, 2020, 26(7):35-40, 25. doi:10.19870/j.cnki.11-3716/ts.2020.07.010. doi: 10.19870/j.cnki.11-3716/ts.2020.07.010
	Wang S S, Liu J M, Sun J, Jia N, Fan B, Dai X F. Research progress on endophytes of medicinal Dendrobium[J]. Food and Nutrition in China, 2020, 26(7):35-40, 25.
[9]	潘超美, 贺红, 林群英, 关则婷. 真菌诱导子对铁皮石斛组培物生长的影响[J]. 中医药学刊, 2004, 22(1):54-55. doi:10.13193/j.archtcm.2004.01.53.panchm.027. doi: 10.13193/j.archtcm.2004.01.53.panchm.027
	Pan C M, He H, Lin Q Y, Guan Z T. Effects of fungal elicitors on the growth of Dendrobium candidum materials[J]. Study Journal of Traditional Chinese Medicine, 2004, 22(1):54-55.
[10]	邢晓科. 药用植物内生真菌资源--一个亟待开发的宝库[J]. 菌物学报, 2018, 37(1):14-21. doi:10.13346/j.mycosystema.170250. doi: 10.13346/j.mycosystema.170250
	Xing X K. Endophytic fungal resource of medicinal plants-a treasure need to be developed urgently[J]. Mycosystema, 2018, 37(1):14-21.
[11]	李景蕻, 张丽华, 张宇. 中药材铁皮石斛组培苗不同培养基的筛选与优化[J]. 基因组学与应用生物学, 2018, 37(6):2551-2557. doi:10.13417/j.gab.037.002551. doi: 10.13417/j.gab.037.002551
	Li J H, Zhang L H, Zhang Y. Screening and optimization onof different medium ofin Chinese herbal medicine of Dendrobium officinale seedlings[J]. Genomics and Applied Biology, 2018, 37(6):2551-2557.
[12]	郭顺星, 曹文芩, 高微微. 铁皮石斛及金钗石斛菌根真菌的分离及其生物活性测定[J]. 中国中药杂志, 2000, 25(6):338-341. doi:10.3321/j.issn:1001-5302.2000.06.005. doi: 10.3321/j.issn:1001-5302.2000.06.005 pmid: 12512420
	Guo S X, Cao W Q, Gao W W. Isolation and biological activity of mycorrhizal fungi from Dendrobium candidum and D.nobile[J]. China Journal of Chinese Materia Medica, 2000, 25(6):338-341. pmid: 12512420
[13]	陈瑞蕊, 施亚琴, 林先贵, 张宁, 汤卫国. 兰科菌根真菌对石斛组培苗的接种效应[J]. 土壤, 2004, 36(6):658-661. doi:10.3321/j.issn:0253-9829.2004.06.014. doi: 10.3321/j.issn:0253-9829.2004.06.014
	Chen R R, Shi Y Q, Lin X G, Zhang N, Tang W G. Effect of inoculation of orchid mycorrhizal fungi on growth of tissue-cultured Dendrobium seedlings[J]. Soils, 2004, 36(6):658-661.
[14]	李景蕻, 张丽华, 邓樱花, 华丽, 郑新春, 李春涵. 铁皮石斛组培苗菌根真菌接种效应[J]. 基因组学与应用生物学, 2019, 38(9):4135-4141. doi:10.13417/j.gab.038.004135. doi: 10.13417/j.gab.038.004135
	Li J H, Zhang L H, Deng Y H, Hua L, Zheng X C, Li C H. Inoculation effect of mycorrhizal fungi in tissue culture seedlings of Dendrobium officinale[J]. Genomics and Applied Biology, 2019, 38(9):4135-4141.
[15]	柯海丽, 宋希强, 谭志琼, 刘红霞, 罗毅波. 野生五唇兰根部内生真菌多样性研究[J]. 生物多样性, 2007, 15(5):456-462. doi:10.3321/j.issn:1005-0094.2007.05.002. doi: 10.1360/biodiv.070060
	Ke H L, Song X Q, Tan Z Q, Liu H X, Luo Y B. Endophytic fungi diversity in root of Doritis pulcherrima(Orchidaceae)[J]. Biodiversity Science, 2007, 15(5):456-462. doi: 10.1360/biodiv.070060 URL
[16]	常美花, 金亚征, 王莉. 铁皮石斛快繁技术体系研究[J]. 中草药, 2012, 43(7):1412-1417.
	Chang M H, Jin Y Z, Wang L. Rapid propagation technique system of Dendrobium officinale[J]. Chinese Traditional and Herbal Drugs, 2012, 43(7):1412-1417.
[17]	付传明, 赵志国, 黄宁珍, 何金祥, 唐凤鸾, 石云平. 铁皮石斛无菌播种产业化繁育技术研究[J]. 广西植物, 2012, 32(2):238-242, 273. doi:10.3969/j.issn.1000-3142.2012.02.018. doi: 10.3969/j.issn.1000-3142.2012.02.018
	Fu C M, Zhao Z G, Huang N Z, He J X, Tang F L, Shi Y P. Study on technology of aseptic sowing and rapid propagation of Demdrobium officinale[J]. Guihaia, 2012, 32(2):238-242, 273.
[18]	殷丽青, 孙翊, 周音, 张永春, 朱建军, 杨柳燕, 李水根, 范晓芬. 添加物和植物生长调节剂对铁皮石斛离体快速繁殖的影响[J]. 上海交通大学学报(农业科学版), 2014, 32(4):8-13, 23. doi:10.3969/J.ISSN.1671-9964.2014.04.002. doi: 10.3969/J.ISSN.1671-9964.2014.04.002
	Yin L Q, Sun Y, Zhou Y, Zhang Y C, Zhu J J, Yang L Y, Li S G, Fan X F. Effects of additives and plant growth regulators on in vitro propagation in Dendrobium candidum[J]. Journal of Shanghai Jiaotong University (Agricultural Science), 2014, 32(4):8-13, 23.
[19]	李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000:134-137.
	Li H S. Plant physiology and biochemistry experiment principle and technology[M]. Beijing: Higher Education Press, 2000:134-137.
[20]	陈金花, 王存, 石蕾, 尹俊梅. 六种真菌诱导子对石斛兰088组培苗生长的影响[J]. 湖南农业科学, 2014(3):28-31. doi:10.16498/j.cnki.hnnykx.2014.03.015. doi: 10.16498/j.cnki.hnnykx.2014.03.015
	Chen J H, Wang C, Shi L, Yin J M. Effects of six kinds of fungal elicitors on growth of Dendrobium nobile 088 tissue culture seedlings[J]. Hunan Agricultural Sciences, 2014(3):28-31.
[21]	龚记熠, 刘杰, 李菲, 孙威, 张习敏, 乙引. 内生真菌对干旱胁迫下金钗石斛生理生化特性的影响[J]. 安徽农业大学学报, 2018, 45(1):156-160. doi:10.13610/j.cnki.1672-352x.20180302.001. doi: 10.13610/j.cnki.1672-352x.20180302.001
	Gong J Y, Liu J, Li F, Sun W, Zhang X M, Yi Y. Effects of endophytic fungi on physiological and biochemical characteristics of Dendrobium nobile under drought stress[J]. Journal of Anhui Agricultural University, 2018, 45(1):156-160.
[22]	徐文婷, 张雅琼, 董文汉, 白燕冰, 李泽生, 孙文丽, 杨生超, 谢世清, 梁泉. 石斛内生真菌固体菌剂对铁皮石斛组培苗促生作用研究[J]. 西南农业学报, 2014, 27(1):317-324. doi:10.16213/j.cnki.scjas.2014.01.053. doi: 10.16213/j.cnki.scjas.2014.01.053
	Xu W T, Zhang Y Q, Dong W H, Bai Y B, Li Z S, Sun W L, Yang S C, Xie S Q, Liang Q. Growth promoting of Dendrobium endophytic fungus on Dendrobium officinale tissue culture seedlings[J]. Southwest China Journal of Agricultural Sciences, 2014, 27(1):317-324.

处理 Treatment	平均鲜质量增长率/% Average increase rate of fresh weight	苗生长状况 Growth status of seedlings
处理 Treatment	平均鲜质量增长率/% Average increase rate of fresh weight	每株叶片数 Number of leaves per plant	茎粗/cm Stem diameter	株高/cm Plant height	健壮度 Haleness status
CK	26.2±2.25Cd	3.5±0.31BCb	0.33±0.02Cc	4.62±0.34CDcd	^*
PS1	41.9±2.12Ab	4.5±0.26Aa	0.44±0.05ABb	5.47±0.36ABb	^***
PS2	33.6±1.60Bc	4.2±0.35ABa	0.38±0.02BCbc	5.16±0.25BCbc	^**
PS3	45.9±1.90Aa	4.7±0.42Aa	0.52±0.04Aa	6.15±0.17Aa	^***
PS4	18.4±2.03De	3.1±0.35Cbc	0.36±0.05BCc	4.21±0.46Dd	^*
PS5	15.3±1.07De	2.8±0.21Cc	0.35±0.02BCc	4.87±0.43BCDc	^*

处理 Treatment	平均鲜质量增长率/% Average increase rate of fresh weight	苗生长状况 Growth status of seedlings
处理 Treatment	平均鲜质量增长率/% Average increase rate of fresh weight	每株叶片数 Number of leaves per plant	茎粗/cm Stem diameter	株高/cm Plant height	健壮度 Haleness status
CK	26.2±2.25Cd	3.5±0.31BCb	0.33±0.02Cc	4.62±0.34CDcd	^*
PS1	41.9±2.12Ab	4.5±0.26Aa	0.44±0.05ABb	5.47±0.36ABb	^***
PS2	33.6±1.60Bc	4.2±0.35ABa	0.38±0.02BCbc	5.16±0.25BCbc	^**
PS3	45.9±1.90Aa	4.7±0.42Aa	0.52±0.04Aa	6.15±0.17Aa	^***
PS4	18.4±2.03De	3.1±0.35Cbc	0.36±0.05BCc	4.21±0.46Dd	^*
PS5	15.3±1.07De	2.8±0.21Cc	0.35±0.02BCc	4.87±0.43BCDc	^*

处理 Treatment	根增加率/% Root increase rate	每株根数 Number of rooting per plant	根长/cm Root length	株高/cm Plant height	每株叶片数 Number of leaves per plant	每株分蘖数 Number of tillers per plant
CK	44.2±1.97Cc	4.2±0.38Dc	4.4±0.40Bc	5.8±0.33Bbc	4.3±0.36Cb	3.0±0.32Bb
PS1	88.6±2.45Aa	6.2±0.36ABa	7.2±0.53Aa	7.1±0.42Aa	5.7±0.28ABa	4.4±0.16Aa
PS2	87.8±2.17Aa	6.5±0.33Aa	6.9±0.60Aa	7.3±0.61Aa	5.5±0.44ABa	4.2±0.10Aa
PS3	74.4±1.56Bb	5.3±0.31Cb	5.9±0.30Ab	6.5±0.48ABab	4.6±0.32BCb	4.1±0.30Aa
PS4	40.4±2.02Cc	3.8±0.20Dc	4.5±0.60Bc	5.5±0.20Bc	4.1±0.29Cb	3.0±0.10Bb
PS5	77.6±2.21Bb	5.6±0.32BCb	6.7±0.31Aab	6.7±0.38ABa	5.9±0.46Aa	4.7±0.49Aa

处理 Treatment	根增加率/% Root increase rate	每株根数 Number of rooting per plant	根长/cm Root length	株高/cm Plant height	每株叶片数 Number of leaves per plant	每株分蘖数 Number of tillers per plant
CK	44.2±1.97Cc	4.2±0.38Dc	4.4±0.40Bc	5.8±0.33Bbc	4.3±0.36Cb	3.0±0.32Bb
PS1	88.6±2.45Aa	6.2±0.36ABa	7.2±0.53Aa	7.1±0.42Aa	5.7±0.28ABa	4.4±0.16Aa
PS2	87.8±2.17Aa	6.5±0.33Aa	6.9±0.60Aa	7.3±0.61Aa	5.5±0.44ABa	4.2±0.10Aa
PS3	74.4±1.56Bb	5.3±0.31Cb	5.9±0.30Ab	6.5±0.48ABab	4.6±0.32BCb	4.1±0.30Aa
PS4	40.4±2.02Cc	3.8±0.20Dc	4.5±0.60Bc	5.5±0.20Bc	4.1±0.29Cb	3.0±0.10Bb
PS5	77.6±2.21Bb	5.6±0.32BCb	6.7±0.31Aab	6.7±0.38ABa	5.9±0.46Aa	4.7±0.49Aa

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