Effects of Growth and Cannabinoid Content of Cannabis sativa L.During Reproductive Stage under Different Light Duration

doi:10.7668/hbnxb.20193311

Abstract

Abstract:

In order to investigate the effects of different light duration on reproductive growth and accumulation of main secondary metabolites of C.sativa,using the female cutting population of cultivar Long 6 as experimental material,the plant height,pitch number,effective branching number,leaf area,yield as well as the contents of CBD,THC,CBDA and CBDV for 28 days were observed and recorded.The results showed a significant increase in plant height,leaf area,pitch number with respect light duration,and all these four indexes correlated positively with the light duration.CBD,CBDV and THC showed susceptibility to the change of light duration.The variation trend of these three cannabinoids was consistent after the light duration treatment,and which also consistently kept rising until harvest.In contrast,CBDA decreased after light duration processing.Among the different duration time used,12 h light duration was the most suitable for the growth of C.sativa having had the highest accumulation of CBD,CBDV and THC.This experiment confirmed duration of light as an important factor limiting growth and development of C.sativa and its medicinal quality.This has so far increased our understanding on the regulation of the light environment of C.sativa.

Key words: Cannabis sativa L., Light duration, Reproductive stage, Yield, Cannabinoid content

ZHANG Jing, TANG Qing, DENG Canhui, CHENG Chaohua, DAI Zhigang, CHEN Xiaojun, SU Jianguang, XU Ying. Effects of Growth and Cannabinoid Content of Cannabis sativa L.During Reproductive Stage under Different Light Duration[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(S1): 178-185. doi: 10.7668/hbnxb.20193311.

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References 37

[1]	颜安, 吴敏洁, 甘银波. 光照和温度调控种子萌发的分子机理研究进展[J]. 核农学报, 2014, 28(1):52-59. doi:10.11869/j.issn.100-8551.2014.01.0052. doi: 10.11869/j.issn.100-8551.2014.01.0052
	Yan A, Wu M J, Gan Y B. Progress on regulatory mechanisms of light and temperature on seed germination[J]. Journal of Nuclear Agricultural Sciences, 2014, 28(1):52-59.
[2]	万友名, 马宏, 赵振刚, 李太强, 刘雄芳, 刘秀贤, 李正红. 不同光周期下馥郁滇丁香香妃的成花反应以及花芽分化进程的解剖学研究[J]. 西北植物学报, 2018, 38(9):1659-1666. doi:10.7606/j.issn.1000-4025.2018.09.1659. doi: 10.7606/j.issn.1000-4025.2018.09.1659
	Wan Y M, Ma H, Zhao Z G, Li T Q, Liu X F, Liu X X, Li Z H. Flowering response and anatomical study on process of flower bud differentiation for Luculia gratissima Xiangfei' under different photoperiods[J]. Acta Botanica Boreali-Occidentalia Sinica, 2018, 38(9):1659-1666.
[3]	Okazawa T, Nishijima T. Effect of low light intensity on longevity of flowering on bedding plants targeted for indoor use[J]. Japan Agricultural Research Quarterly, 2017, 51(3):279-286. doi:10.6090/jarq.51.279. doi: 10.6090/jarq.51.279 URL
[4]	单幼霞. 光胁迫对枇杷果实发育期间生理生化的影响[D]. 杭州: 浙江农林大学, 2018.
	Shan Y X. The effect of light stress on physiological and biochemical characteristics on loquat during fruit development[D]. Hangzhou: Zhejiang A&F University, 2018.
[5]	杨有新, 王峰, 蔡加星, 喻景权, 周艳虹. 光质和光敏色素在植物逆境响应中的作用研究进展[J]. 园艺学报, 2014, 41(9):1861-1872. doi:10.16420/j.issn.0513-353x.2014.09.002. doi: 10.16420/j.issn.0513-353x.2014.09.002
	Yang Y X, Wang F, Cai J X, Yu J Q, Zhou Y H. Recent advances in the role of light quality and phytochrome in plant defense resistance against environmental stresses[J]. Acta Horticulturae Sinica, 2014, 41(9):1861-1872.
[6]	Anja Dieleman J, Marjolein Kruidhof H, Weerheim K, Leiss K. LED lighting strategies affect physiology and resilience to pathogens and pests in eggplant(Solanum melongena L.)[J]. Frontiers in Plant Science, 2021, 11:610046. doi:10.3389/fpls.2020.610046. doi: 10.3389/fpls.2020.610046
[7]	王建平, 王纪章, 周静, 贺通, 李萍萍. 光照对农林植物生长影响及人工补光技术研究进展[J]. 南京林业大学学报(自然科学版), 2020, 44(1):215-222. doi:10.3969/j.issn.1000-2006.201806033. doi: 10.3969/j.issn.1000-2006.201806033
	Wang J P, Wang J Z, Zhou J, He T, Li P P. Recent progress of artificial lighting technique and effect of light on plant growth[J]. Journal of Nanjing Forestry University (Natural Sciences Edition), 2020, 44(1):215-222.
[8]	梁颖, 石玉, 靳琇, 张思瑜, 侯雷平, 张毅. 低磷胁迫下光质对番茄幼苗生长及生理抗性的影响[J]. 中国农学通报, 2020, 36(1):56-61.
	Liang Y, Shi Y, Jin X, Zhang S Y, Hou L P, Zhang Y. Effects of light quality on the growth and physiological resistance of tomato seedlings under low-phosphate stress[J]. Chinese Agricultural Science Bulletin, 2020, 36(1):56-61.
[9]	董飞, 王传增, 张现征, 秦瑜, 刘世琦. 不同光质对樱桃番茄幼苗生理与光合特性的影响[J]. 植物生理学报, 2017, 53(7):1208-1214. doi:10.13592/j.cnki.ppj.2017.0060. doi: 10.13592/j.cnki.ppj.2017.0060
	Dong F, Wang C Z, Zhang X Z, Qin Y, Liu S Q. Effects of different light qualities on physiological and photosynthetic characteristics of cherry tomato seedlings[J]. Plant Physiology Journal, 2017, 53(7):1208-1214.
[10]	刘庆, 连海峰, 刘世琦, 孙亚丽, 于新会, 郭会平. 不同光质LED光源对草莓光合特性、产量及品质的影响[J]. 应用生态学报, 2015, 26(6):1743-1750. doi:10.13287/j.1001-9332.20150331.025. doi: 10.13287/j.1001-9332.20150331.025
	Liu Q, Lian H F, Liu S Q, Sun Y L, Yu X H, Guo H P. Effects of different LED light qualities on photosynthetic characteristics,fruit production and quality of strawberry[J]. Chinese Journal of Applied Ecology, 2015, 26(6):1743-1750.
[11]	陈祥伟, 刘世琦, 王越, 刘景凯, 冯磊. 不同LED光源对乌塌菜生长、光合特性及营养品质的影响[J]. 应用生态学报, 2014, 25(7):1955-1962. doi:10.13287/j.1001-9332.20140421.003. doi: 10.13287/j.1001-9332.20140421.003
	Chen X W, Liu S Q, Wang Y, Liu J K, Feng L. Effects of different LED light qualities on growth,photosynthetic characteristics and nutritional quality of savoy[J]. Chinese Journal of Applied Ecology, 2014, 25(7):1955-1962.
[12]	Aalifar M, Aliniaeifard S, Arab M, Zare Mehrjerdi M, Dianati Daylami S, Serek M, Woltering E, Li T. Blue light improves vase life of carnation cut flowers through its effect on the antioxidant defense system[J]. Frontiers in Plant Science, 2020, 11:511. doi:10.3389/fpls.2020.00511. doi: 10.3389/fpls.2020.00511 pmid: 32670299
[13]	陈冰星, 王晓倩, 刘涛, 王苏苏, WANG-PRUSKI Gefu, 赖钟雄, 郭容芳. 不同光质光周期对樱桃萝卜生长发育及营养品质的影响[J]. 西北植物学报, 2020, 40(1):77-86. doi:10.7606/j.issn.1000-4025.2020.01.0077. doi: 10.7606/j.issn.1000-4025.2020.01.0077
	Chen B X, Wang X Q, Liu T, Wang S S, Wang P G, Lai Z X, Guo R F. Effect of different light quality and photoperiods on growth development and nutritional quality of cherry radish[J]. Acta Botanica Boreali-Occidentalia Sinica, 2020, 40(1):77-86.
[14]	张欢, 章丽丽, 李薇, 邢泽南, 张丹, 崔瑾. 不同光周期红光对油葵芽苗菜生长和品质的影响[J]. 园艺学报, 2012, 39(2):297-304. doi:10.16420/j.issn.0513-353x.2012.02.015. doi: 10.16420/j.issn.0513-353x.2012.02.015
	Zhang H, Zhang L L, Li W, Xing Z N, Zhang D, Cui J. Effects of photoperiod under red LED on growth and quality of sunflower sprouts[J]. Acta Horticulturae Sinica, 2012, 39(2):297-304.
[15]	陆思宇, 杨再强. 光周期对切花菊生长及开花的调控[J]. 中国农业气象, 2021, 42(7):596-605. doi:10.3969/j.issn.1000-6362.2021.07.006. doi: 10.3969/j.issn.1000-6362.2021.07.006
	Lu S Y, Yang Z Q. Regulation of photoperiod on the growth and flowering of cut Chrysanthemum[J]. Chinese Journal of Agrometeorology, 2021, 42(7):596-605.
[16]	扶雅芬. 工业大麻叶提取物的抑菌活性及其作用机理[D]. 北京: 中国农业科学院, 2021.
	Fu Y F. The antibacterial activity and its principle of the leaf extract from industrial hemp[D]. Beijing: Chinese Academy of Agricultural Sciences, 2021.
[17]	常丽, 李建军, 黄思齐, 唐慧娟, 陈安国, 张翠萍, 赵立宁, 李德芳. 植物大麻活性成分及其药用研究概况[J]. 生命的化学, 2018, 38(2):273-280. doi:10.13488/j.smhx.20180215. doi: 10.13488/j.smhx.20180215
	Chang L, Li J J, Huang S Q, Tang H J, Chen A G, Zhang C P, Zhao L N, Li D F. A review of active ingredients from Cannabis sativa L.and their medicinal potential[J]. Chemistry of Life, 2018, 38(2):273-280.
[18]	郭孟璧, 陈璇, 郭鸿彦, 郭蓉, 吕品, 杨明. 不同光质对工业大麻生长及其抗癫痫成分大麻二酚积累的影响[J]. 中药材, 2019, 42(10):2220-2225. doi:10.13863/j.issn1001-4454.2019.10.002. doi: 10.13863/j.issn1001-4454.2019.10.002
	Guo M B, Chen X, Guo H Y, Guo R, Lü P, Yang M. Effects of different light quality on growth of industrial Cannabis sativa and accumulation of cannabidiol,an anti-epilepsy compound[J]. Journal of Chinese Medicinal Materials, 2019, 42(10):2220-2225.
[19]	Islam M J, Ryu B R, Azad M O K, Rahman M H, Cheong E J, Lim J D, Lim Y S. Cannabinoids accumulation in hemp(Cannabis sativa L.)plants under LED light spectra and their discrete role as a stress marker[J]. Biology, 2021, 10(8):710. doi:10.3390/biology10080710. doi: 10.3390/biology10080710 URL
[20]	Wei X Y, Zhao X L, Long S H, Xiao Q M, Guo Y, Qiu C S, Qiu H J, Wang Y F. Wavelengths of LED light affect the growth and cannabidiol content in Cannabis sativa L[J]. Industrial Crops and Products, 2021, 165:113433. doi:10.1016/j.indcrop.2021.113433. doi: 10.1016/j.indcrop.2021.113433
[21]	Lalge A, Cerny P, Trojan V. The effects of red,blue and white light on the growth and development of Cannabis sativa L.[C]. Brno-Proceedings of 24th International PhD Students Conference,646-651.
[22]	Rodriguez-Morrison V, Llewellyn D, Zheng Y B. Cannabis yield,potency,and leaf photosynthesis respond differently to increasing light levels in an indoor environment[J]. Frontiers in Plant Science, 2021, 12:646020. doi:10.3389/fpls.2021.646020. doi: 10.3389/fpls.2021.646020
[23]	辛培尧, 何承忠, 孙正海, 杨明, 郭鸿彦. 短日照处理对大麻开花及性别表达的影响[J]. 湖北农业科学, 2008, 47(7):776-778. doi:10.14088/j.cnki.issn0439-8114.2008.07.019. doi: 10.14088/j.cnki.issn0439-8114.2008.07.019
	Xin P Y, He C Z, Sun Z H, Yang M, Guo H Y. Effect of short daylight treatment on inflorescence and sex expression in Cannabis sativa L.[J]. Hubei Agricultural Sciences, 2008, 47(7):776-778.
[24]	Hall J, Bhattarai S P, Midmore D J. The effects of photoperiod on phenological development and yields of industrial hemp[J]. Journal of Natural Fibers, 2014, 11(1):87-106. doi:10.1080/15440478.2013.846840. doi: 10.1080/15440478.2013.846840 URL
[25]	Moher M, Jones M, Zheng Y B. Photoperiodic response of in vitro Cannabis sativa plants[J]. HortScience, 2021, 56(1):108-113. doi:10.21273/hortsci15452-20. doi: 10.21273/hortsci15452-20 URL
[26]	粟建光, 戴志刚. 大麻种质资源描述规范和数据标准[M]. 北京: 中国农业出版社, 2006.
	Su J G, Dai Z G. Descriptors and data standard for hemp(Cannabis sativa L.)[M]. Beijing: China Agriculture Press, 2006.
[27]	Milay L, Berman P, Shapira A, Guberman O, Meiri D. Metabolic profiling of Cannabis secondary metabolites for evaluation of optimal postharvest storage conditions[J]. Frontiers in Plant Science, 2020, 11:583605. doi:10.3389/fpls.2020.583605. doi: 10.3389/fpls.2020.583605
[28]	Campbell B J, Berrada A F, Hudalla C, Amaducci S, McKay J K. Genotype environment interactions of industrial hemp cultivars highlight diverse responses to environmental factors[J]. Agrosystems, Geosciences & Environment, 2019, 2(1):1-11. doi:10.2134/age2018.11.0057. doi: 10.2134/age2018.11.0057
[29]	卢延旭, 董鹏, 崔晓光, 郭建书, 汪岩. 工业大麻与毒品大麻的区别及其可利用价值[J]. 中国药理学通报, 2007, 23(8):1112-1114. doi:10.3321/j.issn:1001-1978.2007.08.031. doi: 10.3321/j.issn:1001-1978.2007.08.031
	Lu Y X, Dong P, Cui X G, Guo J S, Wang Y. Difference between industrial hemp and marijuana hemp and industrial hemp's use value[J]. Chinese Pharmacological Bulletin, 2007, 23(8):1112-1114.
[30]	黄志鹏, 吴海宁, 蒋菁, 贺梁琼, 韩柱强, 钟瑞春, 熊发前, 刘菁, 唐荣华, 唐秀梅. 单粒精播密度对桂花系列花生植株性状、开花及产量和品质的影响[J]. 西南农业学报, 2020, 33(8):1653-1658. doi:10.16213/j.cnki.scjas.2020.8.008. doi: 10.16213/j.cnki.scjas.2020.8.008
	Huang Z P, Wu H N, Jiang J, He L Q, Han Z Q, Zhong R C, Xiong F Q, Liu J, Tang R H, Tang X M. Effects of single seed sowing density on flowering,yield and quality of Guihua series peanut[J]. Southwest China Journal of Agricultural Sciences, 2020, 33(8):1653-1658.
[31]	Happyana N, Agnolet S, Muntendam R, Dam A V, Schneider B, Kayser O. Analysis of cannabinoids in laser-microdissected trichomes of medicinal Cannabis sativa using L cmS and cryogenic NMR[J]. Phytochemistry, 2013, 87:51-59. doi:10.1016/j.phytochem.2012.11.001. doi: 10.1016/j.phytochem.2012.11.001 pmid: 23280038
[32]	陈璇, 张庆滢, 郭蓉, 郭孟璧, 许艳萍, 杨明, 郭鸿彦. 不同发育时期大麻素合成相关酶基因表达特征与大麻素含量的相关分析[J]. 分子植物育种, 2018, 16(2):583-590. doi:10.13271/j.mpb.016.000583. doi: 10.13271/j.mpb.016.000583
	Chen X, Zhang Q Y, Guo R, Guo M B, Xu Y P, Yang M, Guo H Y. Correlation analysis between gene expression characteristics of related enzymes in cannabinoids biosynthesis and cannabinoids content at different developmental stages of Cannabis sativa L.[J]. Molecular Plant Breeding, 2018, 16(2):583-590.
[33]	Shiponi S, Bernstein N. The highs and lows of P supply in medical Cannabis:Effects on cannabinoids,the ionome,and Morpho-physiology[J]. Frontiers in Plant Science, 2021, 12:657323. doi:10.3389/fpls.2021.657323. doi: 10.3389/fpls.2021.657323
[34]	Livingston S J, Quilichini T D, Booth J K, Wong D C J, Rensing K H, Laflamme-Yonkman J, Castellarin S D, Bohlmann J, Page J E, Samuels A L. Cannabis glandular trichomes alter morphology and metabolite content during flower maturation[J]. The Plant Journal:for Cell and Molecular Biology, 2020, 101(1):37-56. doi:10.1111/tpj.14516. doi: 10.1111/tpj.14516 pmid: 31469934
[35]	Bernstein N, Gorelick J, Koch S. Interplay between chemistry and morphology in medical Cannabis(Cannabis sativa L.)[J]. Industrial Crops and Products, 2019, 129:185-194. doi:10.1016/j.indcrop.2018.11.039. doi: 10.1016/j.indcrop.2018.11.039
[36]	Spitzer-Rimon B, Duchin S, Bernstein N, Kamenetsky R. Architecture and florogenesis in female Cannabis sativa plants[J]. Frontiers in Plant Science, 2019, 10:350. doi:10.3389/fpls.2019.00350. doi: 10.3389/fpls.2019.00350 pmid: 31001293
[37]	伍菊仙, 杨明, 郭孟璧, 李建成, 李鸿, 郭鸿彦. 不同栽培措施对大麻酚类物质含量的影响研究[J]. 中国麻业科学, 2010, 32(2):94-98. doi:10.3969/j.issn.1671-3532.2010.02.006. doi: 10.3969/j.issn.1671-3532.2010.02.006
	Wu J X, Yang M, Guo M B, Li J C, Li H, Guo H Y. The study on the effect of different cultivation measures on the contents of cannabinoids[J]. Plant Fiber Sciences in China, 2010, 32(2):94-98.

性状 Character	处理组 Treatment	0 d	7 d	14 d	21 d	28 d
株高增长量/cm	8 h	-	25.64±2.18b	20.20±3.15c	4.49±2.31a	0.68±1.09a
Plant height increase	10 h	-	24.24±2.00b	25.90±4.15b	3.15±1.73a	1.43±0.66a
	12 h	-	33.13±3.75a	31.21±4.51a	4.50±2.71a	1.34±1.10a
节数/个	8 h	16.00±0.93a	19.88±0.64a	23.13±1.13b	24.75±1.04b	-
Pitch number	10 h	15.88±1.25a	19.00±1.60a	23.25±1.91b	26.00±1.93ab	-
	12 h	16.38±1.06a	19.88±1.25a	25.13±1.73a	27.25±1.75a	-
有效分枝数/个	8 h	7.63±1.30a	11.63±1.41a	12.50±1.07a	12.63±1.06a	-
Effective branching	10 h	7.63±1.19a	10.25±1.75a	11.63±2.00a	12.13±1.89a	-
number	12 h	7.50±1.31a	10.25±1.83a	12.13±1.73a	12.13±1.55a	-
叶面积/mm²	8 h	4 100.51±360.76a	3 425.54±418.02b	2 801.81±269.73b	2 337.39±456.54a	-
Leaf area	10 h	4 108.28±966.22a	3 367.48±401.62b	2 937.01±581.49ab	2 544.05±492.43a	-
	12 h	4 000.20±407.44a	4 087.25±550.86a	3 479.03±673.84a	2 302.44±310.83a	-

性状 Character	处理组 Treatment	0 d	7 d	14 d	21 d	28 d
株高增长量/cm	8 h	-	25.64±2.18b	20.20±3.15c	4.49±2.31a	0.68±1.09a
Plant height increase	10 h	-	24.24±2.00b	25.90±4.15b	3.15±1.73a	1.43±0.66a
	12 h	-	33.13±3.75a	31.21±4.51a	4.50±2.71a	1.34±1.10a
节数/个	8 h	16.00±0.93a	19.88±0.64a	23.13±1.13b	24.75±1.04b	-
Pitch number	10 h	15.88±1.25a	19.00±1.60a	23.25±1.91b	26.00±1.93ab	-
	12 h	16.38±1.06a	19.88±1.25a	25.13±1.73a	27.25±1.75a	-
有效分枝数/个	8 h	7.63±1.30a	11.63±1.41a	12.50±1.07a	12.63±1.06a	-
Effective branching	10 h	7.63±1.19a	10.25±1.75a	11.63±2.00a	12.13±1.89a	-
number	12 h	7.50±1.31a	10.25±1.83a	12.13±1.73a	12.13±1.55a	-
叶面积/mm²	8 h	4 100.51±360.76a	3 425.54±418.02b	2 801.81±269.73b	2 337.39±456.54a	-
Leaf area	10 h	4 108.28±966.22a	3 367.48±401.62b	2 937.01±581.49ab	2 544.05±492.43a	-
	12 h	4 000.20±407.44a	4 087.25±550.86a	3 479.03±673.84a	2 302.44±310.83a	-

处理组 Treatment	鲜质量 Fresh weight	干质量 Dry weight
8 h	45.61±9.57c	8.74±1.70c
10 h	67.18±14.98b	13.34±2.54b
12 h	98.45±13.69a	20.85±3.75a

处理组 Treatment	鲜质量 Fresh weight	干质量 Dry weight
8 h	45.61±9.57c	8.74±1.70c
10 h	67.18±14.98b	13.34±2.54b
12 h	98.45±13.69a	20.85±3.75a

项目 Item	株高增长量 Plant height increase	叶面积 Leaf area	节数 Pitch number	有效分枝数 Effective branching number	鲜质量 Fresh weight	干质量 Dry weight
株高增长量 Plant height increase	1.000
叶面积 Leaf area	0.146	1.000
节数 Pitch number	0.508^*	0.017	1.000
有效分枝数 Effective branching number	-0.148	-0.425^*	0.484^*	1.000
鲜质量 Fresh weight	0.682^**	0.256	0.410^*	-0.159	1.000
干质量 Dry weight	0.756^**	0.111	0.538^**	0.121	0.737^**	1.000

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