Abstract:

In order to reveal the diversity characteristics of endophytes in different species and different organs,the correlation between endophyte community structure and host species and organ types was preliminarily clarified.The stems,leaves and leaf sheaths,which were respectively collected from the plants of the foxtail millet blast-susceptible varieties Shawan millet and Jigu 22 and the foxtail millet blast resistant varieties Xiaoqinggu and Shiliuzi,were used to carry out the endophytic diversity by high-throughput sequencing based on the 16S rDNA V3—V4 region.There were certain differences in endophyte species composition between susceptible and resistant varieties.In all tested samples,the dominant groups at the phylum level were Proteobacteria and Actinobacteriota.Bacteroidota,Chloroflexi,Myxococcota,Firmicutes followed.Alpha diversity analysis showed that the susceptible varieties(Shawan millet,Jigu 22)had higher abundance of endophyte in leaves.PCoA analysis revealed that the organ type had a greater impact on the endophyte community structure than the variety.Species composition analysis showed that the susceptible varieties Shawan millet and Jigu 22 contained endophytic flora that were significantly different from those of Xiaoqinggu and pomegranate(resistant to foxtail millet blast).The susceptible varieties(Shawan millet,Jigu 22)contained Entotheonellaeota phylum in leaves,while resistant varieties had Hydrogenedentes phylum in leaf sheaths.It clarified that the diversity and community structure of endophytes in different organs and millet varieties susceptible and resistant to foxtail millet blast were different,and organ types had a greater impact on the community structure of endophytes than varieties.

Key words: Setaria italica, Magnaporthe oryzae, Endogenic bacteria, Diversity