2-DE Analysis of Cold-response Specific Proteins in Cortex of Branches in Peach Tree

doi:10.7668/hbnxb.20191207

Abstract

Abstract: Cold stress is a major environmental factor that limits peach growth,development reproduction and distribution. Clarifying the molecular mechanism of peach in response to low temperature stress could provide the research basis for cultivating and screening relatively cold resistant peach varieties. In cold-tolerant peach cultivar Qiuyan were selected as testing materials. 1 year dormant branches were exposed to-5(the control),-15,-25℃under controlled environment chamber for 48 h respectively. Two-dimensional electrophoresis(2-DE)were used to analyze proteomics of cortex of branches under 3 different low temperature treatments. The results showed that a total of 150 differentially expressed proteins were identified by mass spectrometry,97 significantly up-regulated proteins and 53 significantly down-regulated after cold stress. After further analysis of the protein spots by proteome data retrieval,20 proteins related to cold stress were identified,involving amino sugar and nucleotide sugar metabolism,starch and sucrose metabolism,amino acid metabolism,MAPK signaling pathway,phenylalanine metabolism and DNA replication. In addition,Real-time PCR (RT-PCR) indicated that three candidate protein-encoding genes Alpha-amylase(M5VVU6),Chlorophyll a/b binding protein(M5WUQ0)and Epoxide hydrolase 3(D8L7V9)were up-regulated with the increase of cold stress. It suggested that these metabolic pathways and three specific proteins might play an important role in the cold-resistant mechanism in peach.

Key words: Peach, Two-dimensional electrophoresis (2-DE), Specific proteins, Cold tolerance

CLC Number:

S662.1

LI Yonghong, ZHOU Yuchao, TIAN Qihang, CHANG Ruifeng, LIU Guojian, CHEN Hu, WANG Zhaoyuan. 2-DE Analysis of Cold-response Specific Proteins in Cortex of Branches in Peach Tree[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(S1): 102-108. doi: 10.7668/hbnxb.20191207.

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