Principal Component Analysis and Comprehensive Evaluation on Biochemical Characteristics of Cold Tolerance at Booting Stage in the Near-isogenic Lines from Japonica Rice Lijiangxintuanheigu

doi:10.7668/hbnxb.2014.S1.050

Abstract

Abstract: The biochemical evaluation system were established for screening and identifying species with cold tolerance,105 rice materials,including three standard varieties of strong cold resistance from Yunnan Province,the NILs BC₄F₈ and BC₄F₉ generation derived from parents japonica cultivars Lijiangxintuanheigu(donor parent) and Towada(recurrent parent),and their parents,were employed for evaluation of cold tolerance and screening of cold resistance index at booting stage.26 biochemical traits including leaves,anthers,and grains of tested materials were determined under cold water-stress and normal conditions in 2011,2012.Comprehensive assessment of cold resistance based on the principal component analysis,subordinate function values analysis,regression analysis and K-means clustering analysis.The results showed Chlorophyll a,Chlorophyll b,total Chlorophyll,Chlorophyll c content,peroxidase(POD) activity,free amino acid content of flag leaves;soluble protein,soluble sugar,malondiadehyde(MDA) content,catalase(CAT) activity,ascorbic acid(ASA),proline content(Pro) of flag leaves and anthers;soluble protein,soluble sugar,protein,γ-aminobutyric acid(GABA) content of grains significantly influenced the cold resistance.Thus could be used as comprehensive index for identification of cold resistance at the booting stage.The values cold resistance comprehensive evaluation(D) of biochemical traits including leaves,anthers,and grains of tested materials based on the subordinate function values was chosen as object under cold stress condition,through K-means clustering analysis,105 lines of population should be divided into 3 categories including stongly cold-tolerant lines 32,mediate cold tolerance lines 10,and cold-sensitive lines 63.

Key words: Rice, Near-isogenic lines, Cold tolerance at booting stage, Biochemical identification index, Principal component analysis, Comprehensive evaluation

CLC Number:

S515.03

YANG Shu-ming, ZENG Ya-wen, WANG Li, ZHANG Su-hua, DU Juan, YANG Tao, PU Xiao-ying, YANG Xiao-meng. Principal Component Analysis and Comprehensive Evaluation on Biochemical Characteristics of Cold Tolerance at Booting Stage in the Near-isogenic Lines from Japonica Rice Lijiangxintuanheigu[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(S1): 262-272. doi: 10.7668/hbnxb.2014.S1.050.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2014.S1.050

http://www.hbnxb.net/EN/Y2014/V29/IS1/262

References

[1] Zhou L,Zeng Y W,Zheng W W,et al.Fine mapping a QTL qCTB7 for cold tolerance at the booting stage on rice chromosome 7 using a near-isogenic line[J].Theoretical and Applied Genetics,2010,121(5):895-905.
[2] Kuroki M,Saito K,Matsuba S,et al.A quantitative trait locus for cold tolerance at the booting stage on rice chromosome 8[J].Theoretical and Applied Genetics,2007,115(5):593-600.
[3] Farrell T C,Fox K M,Lewin L G.Minimising cold damage during reproductive development among temperate rice genotypes.II.Genotypic variation and flowering traits related to cold tolerance screening[J].Australian Journal Agricultural Research,2006,57:89-100.
[4] Zhou L,Zeng Y W,Hu G L,et al.Characterization and identification of cold tolerant near-isogenic lines in rice( Oryza sativa L.)[J].Breeding Science,2012,62:196-201.
[5] Zeng Y W,Yang S M,Cui H,et al.QTLs of cold Tolerance-related traits at the booting stage for NIL-RILs in rice revealed by SSR[J].Genes & Genomics,2009,31(2):143-154.
[6] Oliver S N,Dongen J T,Alfred S C,et al.Cold-induced repression of the rice anther-specific cell wall invertase gene OSINV4 is correlated with sucrose accumulation and pollen sterility[J].Plant Cell and Environment,2005,28(12):1534-1551.
[7] 邓化冰,车芳璐,肖应辉,等.开花期低温胁迫对水稻花粉性状及剑叶理化特性的影响[J].应用生态学报,2011,22(1):66-72.
[8] 范淑秀,王嘉宇,毛艇,等.水稻孕穗期叶绿素含量的QTL定位[J].华北农学报,2010,25(4):69-72.
[9] Theocharis A,Clement C,Barka E A.Physiological and molecular changes in plants grown at low temperatures[J].Planta,2012,235(6):1091-1105.
[10] 阳标仁,余显权,赵福胜,等.水稻孕穗期抗冷鉴定指标选择[J].山地农业生物学报,2008,27(2):101-104,122.
[11] Fernandez O,Theocharis A,Bordiec S,et al.Burkholderia phytofirmans strain PsJN acclimates grapevine to cold by modulating carbohydrates metabolism[J].Molecuar Plant Microbe Interact,2012,25:496-504.
[12] 谢勇武,杨树明,曾亚文,等.粳稻02428×02428c重组自交系孕穗期耐冷性QTL分析[J].中国水稻科学,2011,25(6):681-684.
[13] 戴陆园,叶昌荣,熊建华,等.水稻耐冷性鉴定评价方法[J].中国水稻科学,1999,12(1):62-67.
[14] Jiang W,Lee J,Chu S H,et al.Genotype x environment interactions for chilling tolerance of rice recombinant inbred lines under different low temperature environments[J].Field Crops Research,2010,117,226-236.
[15] Theocharis A,Bordiec S,Fernandez O,et al.Burkholderia phytofirmans strain PsJN primes Vitis vinifera L.and confers a better tolerance to low non-freezing temperatures[J].Molecular Plant Microbe Interact,2011,25:241-249.
[16] Horisue N,Kunihiro Y,Higashi T,et al.Screening for cold tolerance of Chinese and Japanese rice varieties and selection of standard varieties[J].Tropical Agricultural Research Service,1988,21:76-87.
[17] 杨树明,王荔,曾亚文,等.粳稻丽江新团黑谷近等基因系孕穗期耐冷性指标性状的遗传分析[J].华北农学报,2013,28(1):7-11.
[18] 张志良,瞿伟菁.植物生理学实验指导[M].北京:高等教育出版社,2003.
[19] 郝建军,刘延吉.植物生理学试验技术[M].沈阳:辽宁科学技术出版社,2001.
[20] 邹琦.植物生理学实验指导[M].北京:中国农业出版社,2000.
[21] 李合生,孙群,赵世杰,等.植物生理生化实验原理与技术[M].北京:高等教育出版社,2000:184-185.
[22] 董晓燕.生物化学实验[M].北京:化学工业出版社,2003:34-37.
[23] Inatomi K,Slaughter J C.The role of glutamate decarbozylase and γ-aminobutyric acid in germinating barly[J].Journal of Experimental Botony,1971,22:561-571.
[24] 陈能,罗玉坤,谢黎虹,等.我国水稻品种的蛋白质含量及与米质的相关性研究[J].作物学报,2006,32(8):1193-1196.
[25] 蒋志农.云南稻作[M].昆明:云南科技出版社,1995.
[26] 钟楚,朱颖墨,朱勇,等.云南不同类型一季稻产量形成及其与气象因子的关系[J].应用生态学报,2013,24(10):2831-2842.
[27] 姜丽霞,季生太,李帅,等.黑龙江省水稻空壳率与孕穗期低温的关系[J].应用生态学报,2010,21(7):1725-1730.
[28] Ruelland E,Zachowski A.How plants sense temperature[J].Environmental and Experimental Botany,2010,69(3):225-232.
[29] Uemura M,Warren G,Steponkus P L.Freezing sensitivity in the sfr4 mutant of Arabidopsis is due to low sugar content and is manifested by loss of osmotic responsiveness[J].Plant Physiology,2003,131(4):1800-1807.
[30] Suzuki N,Koussevitzky S,Mittler R,et al.ROS and redox signalling in the response of plants to abiotic stress[J].Plant Cell Environment,2012,35(2):259-270.
[31] 杨树明.强耐冷稻种丽江新团黑谷近等基因系孕穗期耐冷性及QTLs分析[D].昆明:云南农业大学,2014.

Please choose a citation manager

Content to export