Estimation of Genome Size of Apricots Based on Flow Cytometry

doi:10.7668/hbnxb.20191226

Abstract

Abstract: Genome size is the critical parameter of biodiversity characteristics in plants. Some valuable data, including species evolution and classification studies and genomic research in apricot, will be acquired when performed the apricot genome size and comparing the difference among species, varieties and cultivars. The genome size of 13 apricot cultivars belonged to five species had been detected by using flow cytometry. The five species of apricot included P. armeniaca, P. sibirica, P. mandshurica, P. mume and P. dasycarpa. After comparing three internal reference plants, Z. jujuba cv. Jinsixiaozao was used as an internal reference and the DNA content was quantified by flow cytometry and calculated as the ratio of mean flurescence of G0/G1 peak between Z. jujuba and tested samples. The genome size of apricot and its variants varied between (253.17±0.963)Mb (Dayoujia)-(300.60±1.181)Mb (Shanmei), Zixing had the most small genome size. The genome sizes of Liaoxing, Lüeshanxing belonging to P. sibirica, and Longwangmao were (280.37±4.858), (273.66±4.334),(263.42±1.306)Mb, respectively. There were significant difference in genome size among the five different species and varieties of the apricot genus. This study detected the difference in genome size among different species and varieties in apricot for the first time. These results would provide basic evidence for exploring the relationship between the genome sizes and their phenotypic traits, interspecies differentiation and botanical classification in apricot.

Key words: Apricot, Genome size, Flow cytometry, Estimation

CLC Number:

S602.4

ZHANG Junhuan, YANG Li, JIANG Fengchao, ZHANG Meiling, SUN Haoyuan, WANG Yuzhu. Estimation of Genome Size of Apricots Based on Flow Cytometry[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(5): 32-38. doi: 10.7668/hbnxb.20191226.

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