Abstract: The C-repeat binding factors(CBF)/dehydration-responsive element-binding protein(DREB) are a group of conserved transcription factors that play an important role in the response and adaptation to environmental stress in many plants. In order to investigate the role of CBF transcription factors in plant abiotic stresses of Prunus armeniaca L.,a CBF/DREB1 transcription factor gene was cloned using reverse transcription-PCR(RT-PCR) from Weixuan No.1 and designated as PaCBF1. The GenBank accession number was MF491392. Bioinformatics analysis was carried out and the results showed that:The open reading frame(ORF) of PaCBF1 was 723 bp,which encoded a protein of 240 amino acids with a molecular weight of 26.928 ku and a pI of 6.35. The grand average of hydropathicity was -0.555.PaCBF1 protein contained an AP2 DNA-binding domain and two CBF signature sequences that one was PKKRAGRRVFKETRHP and the other was DSAWR. The prediction of secondary structure of PaCBF1 protein indicated that the encoded protein mainly contained 38.75% of random coil,29.58% of α-helix,22.92% of extended strand and 8.75% of β-turn. Tertiary structure of PaCBF1 protein contained α-helix,β-pleated sheet and β-turn. PaCBF1 protein was localized in the nucleus,it did not contain a signal peptide,and it was not a secreted protein. PaCBF1 protein contained 13 phosphorylation sites. These results provided a theoretical basis for the further study of the function of the kernel apricot CBF transcription factor and the resistance mechanism to the stress environment.

Key words: Kernel apricot, PaCBF1, Gene clone, Bioinformatics

CLC Number: 

• Q78

• S662.03