Functional Analysis of Polygalacturonase-inhibiting Protein Gene from Panax notoginseng

doi:10.7668/hbnxb.2018.02.010

Abstract

Abstract: Polygalacturonase-inhibiting protein(PGIP) plays an important role in plant defense system. PnPGIP, a gene encoding a PGIP protein,was isolated from Panax notoginseng(Burk.) F.H. Chen,and responded the infection of Fusarium solani at the transcriptional level. The objective of this paper was to analyze the function of PnPGIP. The prokaryotic expression vector of PnPGIP was constructed and transferred into Escherichia coli BL21(DE3) for a large amount of expression of the recombinant protein,which was purified to perform the in vitro plate antifungal assay. In addition,the plant overexpression vector was constructed and the transgenic tobacco was obtained by Agrobacterium tumefaciens mediated genetic transformation. The resistance of T₂ transgenic tobacco was analyzed in vivo. The in vitro plate antifungal experiments indicated that the recombinant protein strongly inhibited mycelial growth of Colletotrichum gloeosporioides, Sclerotinia sclerotiorum, F.solani and Fusarium verticillioides, moreover,the inhibitory activity increased with the increased of PnPGIP protein. qRT-PCR analysis showed that PnPGIP was highly expressed in T₂ transgenic tobacco. Furthermore,the resistance of transgenic tobacco to F.solani was significantly enhanced compared with wild type tobacco. PnPGIP is an important disease resistance gene involved in defense response of Panax notoginseng to F.solani infection.

Key words: Panax notoginseng (Burk.) F.H. Chen, PGIP, Resistance, Fusarium solani, Functional verification

CLC Number:

S432
Q786

GUAN Ruipan, BAI Zhiwei, WANG Qian, TANG Bifeng, CUI Xiuming, LIU Diqiu. Functional Analysis of Polygalacturonase-inhibiting Protein Gene from Panax notoginseng[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(2): 65-71. doi: 10.7668/hbnxb.2018.02.010.

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