Studies on Ploidy Composing in Regenerated Plants from Anther Culture of Pepper (Capsicum annuum L.)

doi:10.3321/j.issn:1000-7091.2007.01.029

Abstract

Abstract: Flow cytometric (FCM) analytical technique and chromosome counting were used for the detailed ploidy identification in population of anther culture derived pepper plants. Results revealed ploidy diversity in regenerated plants. The different ploidy in organs (eg. Leave and root tips) of the same plant was observed, and this phenomena showed the importance of determining ploidy level by different samples. Chromosome deletion was observed in some cells of haploid or double haploid plants. The coincidence between FCM and chromosome counting was high up to 0.95 in our experiment. We also discussed about the phenomenon of peak distortion in FCM analytical technique. These observations and results are significant for the haploid breeding, germplasm innovation and cytogenetic researches.

Key words: Pepper (Capsicum annuum L.), Anther culture, Ploidy composing, Flow cytometry, Chromosome counting

CLC Number:

Q343

CHEN Bin, ZHAO Hong, GENG San-sheng, ZHANG Bao-xi, ZHANG Yue-yun, LIU Fan. Studies on Ploidy Composing in Regenerated Plants from Anther Culture of Pepper (Capsicum annuum L.)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(1): 123-128. doi: 10.3321/j.issn:1000-7091.2007.01.029.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.3321/j.issn:1000-7091.2007.01.029

http://www.hbnxb.net/EN/Y2007/V22/I1/123

References

[1] Ockendon D J. The ploidy of plants obtained from anther culture of cauliflowers (Brassica oleracea var. botrytis)[J]. Annals of Applied Biology, 1988, 113: 319-325.
[2] Chiang M S, Frechette S, Kuo C G, et al. Embryogenesis and haploid plant production from anther culture of cabbage (Brassica oleracea var. capitata L)[J]. Can J Plant Sci, 1985, 65: 1033-1037.
[3] 李春铃, 蒋钟仁. 甜椒花培新品种"海花三号"的育成[J]. 园艺学报, 1990, 17(1): 39-44.
[4] 王玉英, 郭仲琛, 李春玲, 等. 甜椒花药培养的初步研究[J]. 园艺学报, 1980, 7(1): 34-38.
[5] Farnham M W, Caniglia E J, Thomas C E. Efficient ploidy determination of anther deriver broccoli[J]. Hortscience, 1998, 32(2): 323-327.
[6] 申书兴, 赵前程, 刘世雄, 等. 四倍体大白菜小孢子植株的获得与倍性鉴定[J]. 园艺学报, 1999, 26(4): 232-237.
[7] Qin X, Rotino G L. Chloroplast number in guard cells as ploidy indicator of in vitro-grown androgenic pepper plantlets[J]. Plant Cell Tissue and Organ Culture, 1995, 41: 145-149.
[8] 周伟军, 毛碧增, 唐桂香, 等. 甘蓝型油菜小孢子再生植株染色体倍数检测研究[J]. 中国农业科学, 2002, 35(6): 724-727.
[9] 周元昌, S KENNEDY. 抱子甘蓝花培苗倍性的快速鉴定[J]. 福建农林大学学报(自然科学版), 2002, 31(1): 55-58.
[10] Arumuganathan K, Earle E D. Estimation of nuclear DNA content of plants by flow cytometry[J]. Plant Mol Biol Report, 1991, 9: 229-241.
[11] Mityk J, Andr Slafalvy A, Csill Ry G, et al. Anther-culture response in different genotypes and F1 hybirds of pepper (Capsicum annuum L.)[J]. Plant Breeding, 1995, 114: 78-80.
[12] Ramon Ds, Elisabet C, Agustin H. Androgenesis in Capsicum annuum L. effect of carbohydrate and carbon dioxide enrichment[J]. Hort Sci, 1997, 122(4): 468-475.
[13] Gyulai G, Gemesne J A, Sagi Zs, et al. Double Haploid Development and PCR-analysis of F1 Hybird Derived DH-R2 Paprika(Capsicum annuum L.) Line[J]. Plant Physiol, 2000, 156: 168-174.
[14] Dolezel J, Binarova P, Lucretti S. Analysis of nuclear DNA content in Plant cells by flow cytometry[J]. Biol Plant, 1989. 31: 113-120.
[15] 利容千. 中国蔬菜植物核型研究[M]. 武汉: 武汉大学出版社, 1989.
[16] 张冬青, 顾宏辉, 张尧峰, 等. 双低油菜双72的小孢子培养与植株再生研究[J]. 浙江农业学报, 2003, 15(4): 219-222.
[17] 赵岫云. 大白菜游离小孢子培养技术及育种应用研究[D]. 北京: 中国农业科学院研究生院, 2003.
[18] 朱慧琴. 烟草DH群体构建及其农艺性状遗传参数顾鉡D]. 浙江: 浙江大学, 2003.
[19] 王震星, 张磊. 枣花药培养再生植株及其染色体倍性研究[J]. 北方果树, 1998(2): 5-6.
[20] 刘国民. 花药离体培养中若干问题的研究进展[J]. 海南大学学报(自然科学版), 1994, 12(3): 253-259.
[21] 李俊明编译. 植物组织培养教程[M]. 北京: 中国农业大学出版社, 2002.

Please choose a citation manager

Content to export