Marker-assisted Selection and Application of Blast Resistant Gene Pigm(t) in Rice

doi:10.7668/hbnxb.2016.01.009

Abstract

Abstract: This study was aimed to improve the resistance of Wuyunjing 29196.Blast resistant gene Pigm(t) showed a broad spectrum of strong resistance.Wuyunjing 29196 is an excellent conventional Japonica rice,which has good yielding but sensitive to rice blast.This study was aimed to improve the resistance of Wuyunjing 29196 to blast by MAS and anther culture.GM4,a variety carrying a resistant gene Pigm(t),was used as the donor parent.Two closely linked InDel markers,S95477 and S29742,were employed to select the Pigm(t) gene in each generation.Finally,185 DH (Double haploid) lines were developed from the anthers of BC₂F₁ plants.Among them,82 improved lines with homozygous Pigm(t) were selected.Multiple agronomic traits including grain yield and resistance to rice blast and the rice quality were characterized.Two fine lines,DH036 and DH158 screened out,finally which had increased resistance level and enhanced yield production.More importantly,they had similar plant architecture and other traits.Simultaneously had high resistance to rice blast and quality.The combination of anther culture and marker-assisted selection successfully improved the resistance to rice blast,in a short breeding period.The improved Wuyunjing 29196 with high blast resistance level provided important genetic resources in breeding.

Key words: Rice, Pigm(t), Marker assisted selection, Back cross breeding, Anther culture

CLC Number:

WANG Fei, WANG Liguang, PAN Meiyao, NIU Zhongyi, ZHOU Yong, LIANG Guohua. Marker-assisted Selection and Application of Blast Resistant Gene Pigm(t) in Rice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(1): 51-56. doi: 10.7668/hbnxb.2016.01.009.

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http://www.hbnxb.net/EN/Y2016/V31/I1/51

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