RNA Interference Vector Construction of VRN1 Gene and Obtaining Transgenic Wheat

doi:10.7668/hbnxb.2016.01.010

Abstract

Abstract: In order to study the biological function of VRN1 in wheat developmental process, VRN1 expression patterns were analyzed using fluorescence quantitative RT-PCR in two vernalization characteristic varieties Xinchun No.2 and Jing 841.The results showed that the VRN1 transcription levels increased gradually in Xinchun No.2 leaves and shoot meristems.While the VRN1 transcription levels in Jing 841 leaves showed a wavelike rises,but the expression level in shoot meristems was very low and nearly zero.The VRN1 RNA interference sequence was successfully introduced into vector pFGC5941,and the recombined vector was transferred into the shoot apical meristem of Xinchun No.2 by Agrobacterium-mediated transformation.Transgenic plants were detected by PCR amplification,which could lay a foundation for genetic improvement of wheat developmental characteristics on molecular level and created new wheat germplasm for wheat breeding.

Key words: Wheat, Vernaliazation gene VRN1, Expression, Transformation

CLC Number:

S512.03
Q78

ZHANG Baona, ZHU Cancan, ZHANG Pengyu, WEI Li, WANG Xiang, JIANG Yumei, WANG Tongchao. RNA Interference Vector Construction of VRN1 Gene and Obtaining Transgenic Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(1): 57-62. doi: 10.7668/hbnxb.2016.01.010.

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