Expression of IFN-γ of New Zealand Rabbit in Insect Cells and Its Antiviral Activity

doi:10.7668/hbnxb.2010.03.003

Abstract

Abstract: The cDNA specific for New Zealand rabbit IFN-γ was amplified by reverse transcription polymerase from the rabbit peripheral blood lymphocytes( PBMC) stimulated by Concavadin A and sequenced.The sequencing results indicated that the IFN-γ gene was complete,and the homology of nucleotide and amino acid sequence of rabbit IFN-γ comparing the reference sequence in GenBank are 99.4%-99.6% and 98.8%-99.4%.The rabbit IFN-γ gene then was inserted into the transferring vector pFastBac^TMTMTM5 U/mg．

Key words: Rabbit, IFN-γ, Cloning, Recombinant baculovirus

CLC Number:

Q786

WANG Fang, HU Bo, REN Xue-feng, FAN Zhi-yu, ZHANG Ze-bin, XU Wei-zhong, HE Kong-wang. Expression of IFN-γ of New Zealand Rabbit in Insect Cells and Its Antiviral Activity[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(3): 9-13. doi: 10.7668/hbnxb.2010.03.003.

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