Phylogenetic Analysis and Prokaryotic Expression of NP Gene from Subtype H5N1 of Avian Influenza Virus in Escherichia coli

doi:10.7668/hbnxb.2010.03.005

Abstract

Abstract: The Nucleocapsid Protein ( NP) gene of Avian influenza virus( AIV) subtype H5 was amplified by PCR according to the published sequence, and its molecular evolution was analyzed.The coding sequence of NP was cloned into the prokaryotic vector of pET₃2a to express and obtain the recombinant protein inE． coli.The expressed NP was purified and identified with Western-Blot strategy.and its immunogenicity was further characterized with enzyme linked immunosorbent assay.The result suggested that the cloned NP was evolutionary more similar to others of Henan.and the fusion protein was successfully expressed and mainly existed as soluble bodies,Also, the protein could be specifically recognized by the serum against H5 subtype of AIV．

Key words: Avian influenza virus, Subtype H5, Nucleocapsid protein, Prokaryotic expression

CLC Number:

WU Cheng-ye,, WANG Ai-ping, HAO Hui-fang, SHI Ping-ling, YOU Lei-ming, LI Pei-pei, ZHANG Gai-ping. Phylogenetic Analysis and Prokaryotic Expression of NP Gene from Subtype H5N1 of Avian Influenza Virus in Escherichia coli[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(3): 19-22. doi: 10.7668/hbnxb.2010.03.005.

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