The Effects of LiCl and MSAB on the Wnt/β-Catenin Signaling Pathway, Proliferation,and Differentiation in C2C12 Cells

doi:10.7668/hbnxb.20195994

Abstract

Abstract:

The aim of this study was to investigate the effects of the activator (LiCl) and the inhibitor (MSAB) on the Wnt/β-catenin signaling pathway in C₂C₁₂ cells,as well as to explore how activation or inhibition of this pathway influences myogenic differentiation in C₂C₁₂ cells.This study utilized C₂C₁₂ cells as a model,the cells were treated with different concentrations of LiCl (0,15,and 25 mmol/L) as well as IWR-1 and MSAB (0,15,and 25 μmol/L) to induce differentiation.The expression of proteins related to the classical Wnt signaling pathway and myogenic differentiation cells was detected using Western Blotting technology.The results indicated that a concentration of 15 mmol/L LiCl did not had a significant effect on the β-catenin protein within the Wnt signaling pathway.In contrast,a concentration of 25 mmol/L LiCl promoted the expression of β-catenin,Pax7,MyOG,and MyHC in C₂C₁₂ cells.IWR-1 had no significant effect on β-catenin protein,whereas MSAB at concentrations of 15 and 25 μmol/L exhibited an inhibitory effect on the expression of β-catenin protein.Notably,the inhibition with 15 μmol/L MSAB was particularly pronounced,leading to a marked reduction in the expression levels of β-catenin,Pax7,MyOG,and MyHC in C₂C₁₂ cells.In conclusion,25 mmol/L LiCl and 15 μmol/L MSAB can effectively activate and inhibit the Wnt signaling pathway within C₂C₁₂ cells,thereby influencing myogenic differentiation in these cells.

Key words: Wnt/β-catenin signaling pathway, LiCl, MSAB, C₂C₁₂ cells

CLC Number:

S813.1遗传

WEI Haimei, JIANG Meiqi, LIU Zhongzhi, LI Xin, HU Debao, GUO Yiwen, DING Xiangbin, GUO Hong, ZHANG Linlin. The Effects of LiCl and MSAB on the Wnt/β-Catenin Signaling Pathway, Proliferation,and Differentiation in C₂C₁₂ Cells[J]. Acta Agriculturae Boreali-Sinica, 2026, 41(2): 232-238. doi: 10.7668/hbnxb.20195994.

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http://www.hbnxb.net/EN/Y2026/V41/I2/232

Figures/Tables 6

Fig.1 The impact of activator LiCl on β-catenin A. Detecting the effect of different concentrations of LiCl on β-catenin protein by Western Blotting;B. Quantitative graph of bands in Fig.A.The same letters indicate no significant difference, and different letters indicate a significant difference (P<0.05).The same as Fig.2—6.

Fig.2 Effects of inhibitors IWR-1(A,B)and MSAB(C,D)on β-catenin

Fig.3 The effect of LiCl activation on Wnt/β-catenin signaling and C₂C₁₂ cell proliferation

Fig.4 The effect of LiCl on the activation of the Wnt/β-catenin signaling pathway and C₂C₁₂ cell differentiation

Fig.5 The inhibitory effect of MSAB on the Wnt/β-catenin signaling pathway influences the proliferation of C₂C₁₂ cells

Fig.6 The effect of MSAB on the Wnt/β-catenin signaling pathway and differentiation in C₂C₁₂ cells

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