Abstract: The content of total sugar and total phenols were determined to be 75.20 and 32.80 mg/g,15.75 and 0.57 mg/g respectively in leaves and kernels of tung tree.On that basis,high-quality total RNA of leaves and kernels were respectively extracted by modified TRIzol method and modified CTAB-LiCl method,and the effects of the above methods,Guanidinium idothiocyanate method and NOGEN RNAprep pure kit method were compared.The results showed that not only the OD260/OD280 value of 1.9-2.0 of the kernel of RNA isolated by modified TRIzol method stabilized,but also the bands of 28S rRNA and 18S rRNA were clear and complete,and higher yields was even more important.The total RNA of kernels isolated by modified CTAB-LiCl method were degradation in some degrees,and small molecule in the RNA extracted by Guanidinium idothiocyanate method could not be removed fully.Moreover the total RNA of kernels with DNA and degradation in some degrees could be obtained via NOGEN RNAprep pure kit method.In above four methods,only modified CTAB-LiCl method could extract the higher quality total RNA of leaves of tung tree.For these reasons,modified TRIzol method and modified CTAB-LiCl method could be regarded as preferred method for the RNA isolated respectively from leaves and kernels of tung tree.

Key words: Tung tree, Total RNA isolation Modified, TRIzol method Modified, CTAB-LiCl method, Guanidinium idothiocyanate method

CLC Number: 

• Q75