Molecular Marker -assisted Selection for Development of Yellow-seeded Pol CMS Restorer Lines in Brassica napus L .

doi:10.7668/hbnxb.2009.S1.008

Abstract

Abstract: Marker -assisted selection (MAS)was used in a modified recurrent backcross program to transfer the yellow-seeded gene derived from the stable,pure and spring -type yellow -seeded Brassica napus double haploid (DH) line No .2127 -17 to the semi -winter -type Polima cytoplasmic male sterility (CMS)restorer line Hui5148 -2 for breeding Polima cms restorer lines .In this scheme,142 random amplified polymorphic DNA (RAPD)primers were used to select the yellow -seeded DH lines with high recovery of Hui5148 -2.Then ,SCS1130,a dominant sequence -characterized amplified region (SCAR)marker,and SCA1,a co -dominant cleaved amplified polymorphic sequence (CAPS) marker ,were screened in BC1F1 and BC2F1 segregating progeny to select individuals carrying the yellow seed gene .In order to recover the recurrent parent background and simplify the analysis,88 RAPD primers and 60 amplified fragment length polymorphism (AFLP)primer pair assays were used to select lines with the least genetic distance (GD)to Hui5148 -2 using a two -step selected approach in yellow -seeded BC1F1 and BC2F1 plants .As a result,only 5 elite restorer lines were selected with GD values of 0.015 7 -0 .036 4 to Hui5148 -2 from 9 BC2F2 homozygous lines identified by SCA1 and background analysis.

Key words: Marker-assistedselection(MAS), Brassicanapus, Yellow-seededrestorerlines

CLC Number:

LIUZhi-wen, HANXu, WANGYing, FUTing-dong. Molecular Marker -assisted Selection for Development of Yellow-seeded Pol CMS Restorer Lines in Brassica napus L .[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.7668/hbnxb.2009.S1.008.

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