Identification of a SRAP Markers Linked to Leaf Rust Resistance Gene Lr19 in Wheat

doi:10.7668/hbnxb.2007.04.045

Abstract

Abstract: SRAP analysis was firstly carried on Thatcher, twenty three near-isogenic lines and F2 population of TcLr19×Thatcher to develop molecular markers for leaf rust resistance gene Lr19 in wheat. A marker linked to Lr19 resistance gene was obtained, and was named as M73 with the genetic distance 2.6 cM. The reseach may facilitate establishment of the higher density genetic map, marker-assisted selection breeding program and eventual cloning of Lr19 gene.

Key words: Wheat leaf rust, Resistance gene, Molecular marker, SRAP, Lr19

CLC Number:

S432

LIU Ya-hui, YAN Hong-fei, YANG Wen-xiang, MENG Qing-fang, ZHANG Ting, LIU Da-qun. Identification of a SRAP Markers Linked to Leaf Rust Resistance Gene Lr19 in Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(4): 193-196. doi: 10.7668/hbnxb.2007.04.045.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2007.04.045

http://www.hbnxb.net/EN/Y2007/V22/I4/193

References

[1] Denissen C J M. Influence of race and post infection temperature on two components of partial resistance to wheat leaf in seedings of wheat[J]. Euphytica, 1991, 58 (1): 13-20.
[2] Komer J A. Genetics of resistance to wheat leaf rust[J]. Annu Rev Phytopathol, 1996, 34: 435-455.
[3] Winzeler M, Winzeler H, Keller B. Endopestidase polymorphism and linkage of the EP-Dlc null allele with the Lr19 leaf-rust resistance gene in hexaploid wheat[J]. Plant Breeding, 1995, 114: 24-28.
[4] Autrique E R P, Shingh R P, Tanksley S D, et al. Molecular markers for four leaf rust resistance genes introgressed into wheat from wild relatives[J]. Genome, 1995, 38: 75-83.
[5] 李星, 杨文香, 李亚宁, 等. 小麦抗叶锈病基因Lr19的SSR标记[J]. 中国农业科学, 2005, 38(6): 1156-1159.
[6] Prins R J, Groene W Z, Marais G F. et al. AFLP and STS tagging of Lr19, a gene conferring resistance to leaf rust in wheat[J]. Theoretical and Applied Genetics, 2001, 103: 618-642.
[7] 李星, 杨文香, 李亚宁, 等. 小麦抗叶锈病基因Lr19的AFLP标记[J]. 中国农业科学, 2005, 38(9): 1796-1800.
[8] 文雁成, 王汉中, 沈金雄, 等. 用SRAP标记分析中国甘蓝型油菜品种的遗传多样性和遗传基础[J]. 中国农业科学, 2006, 39(2): 246-256.
[9] 潘俊松, 王刚, 李效尊, 等. 黄瓜SRAP遗传连锁图的构建及始花节位的基因定位[J]. 自然科学进展, 2005, 15(2): 167-171.
[10] 任羽, 王得元, 张银东, 等. 辣椒SRAP-PCR反应体系的建立与优化[J]. 分子植物育种, 2004, 2(5): 689-693.
[11] 林忠旭, 张献龙, 聂以春, 等. 新型标记SRAP在棉花F2分离群体及遗传多样性评价中的适用性分析[J]. 遗传学报, 2004, 31: 622-626.
[12] Seyfarth R, Feuillet C, Schachermary G, et al. Development of a molecular marker for the adult plant leaf rust resistance gene Lr35 in wheat[J]. Theoretical and Applied Genetics, 1999, 99: 554-560.
[13] Roelfs A P. Race specificity and methods of study[M]//Bushnell W R, Roelfs A P. The Cereal Rust Vol 1 Origins, Specificity, Structure and Physiology Orlando: Academic Press, 1984, 2: 131-164.
[14] Gill K S, Lubbers E L, Gill B S, et al. A genetic linkage map of Triticum tauschii (DD) and its relationship to the D genome of bread wheat (AABBDD)[J]. Genome, 1991, 34: 362-374.
[15] Li G, Quiros C F. Sequence-related amplified polymorphism(SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica[J]. Theoretical and Applied Genetics, 2001, 103: 455-461.

Please choose a citation manager

Content to export