Acta Agriculturae Boreali-Sinica ›› 2022, Vol. 37 ›› Issue (2): 201-210. doi: 10.7668/hbnxb.20192637

Special Issue: Wheat Biotechnology

• Resources & Environment·Plant Protection • Previous Articles     Next Articles

Identification of Leaf Rust Resistance Genes in 40 CIMMYT Wheat Lines at Seedling Stage and Adult Plant

WANG Jiarong, DONG Rui, ZHANG Mengyu, GAO Pu, ZHANG Peipei, LI Zaifeng, LIU Daqun   

  1. Biological Control Center of Plant Diseases and Pests of Hebei Province,College of Plant Protection, Hebei Agricultural University,Baoding 071001,China
  • Received:2021-10-21 Published:2022-04-28

40份CIMMYT小麦品系苗期及成株抗叶锈病基因鉴定

王佳荣, 董瑞, 张梦宇, 高璞, 张培培, 李在峰, 刘大群   

  1. 河北省农作物病虫害生物防治工程技术研究中心,河北农业大学 植物保护学院,河北 保定 071001
  • 作者简介:
    作者简介:王佳荣(1997—),女,河北保定人,在读硕士,主要从事分子植物病理学研究。
  • 基金资助:
    河北省高等学校科学研究计划(ZD2021053)

Abstract:

To identify leaf rust resistance genes in 40 wheat materials from the International Maize and Wheat Improvement Center (CIMMYT), combined with pedigree analysis, gene derivation and molecular marker detection, 36 known disease resistance gene vector varieties and 40 wheat materials were vaccinated with 17 physiological species of leaf rust bacteria with different toxicity.By comparing the infected types of the tested wheat lines and the known rust resistance genes,the possible known resistance genes in the tested wheat materials could be postulated.At the same time,12 markers closely linked with known resistance genes were used to detect the 40 CIMMYT lines,and the results could be verified with the gene postulation.Furthermore,40 tested lines were planted in Baoding,Hebei Province and Zhoukou,Henan Province,respectively,and inoculated with mixed Pt races for leaf rust evaluation at adult plant stage in the field.The results showed that 7 wheat lines contained Lr1,Lr10 was found in 9 lines,and each of Lr11 and Lr34 was present in 10 lines.Each of Lr14a,Lr15 and Lr26 was postulated to present in 2,4 and 3 lines,respectively.Each of Lr37 and Lr46 was identified in 22,39 wheat lines,respectively,by using molecular marker detection.In the field test 22 wheat lines showed adult plant resistance to leaf rust.

Key words: Wheat leaf rust, Leaf rust resistance gene, Gene postulation, Adult plant leaf rust resistance, Molecular marker detection

摘要:

为了对40份来自国际玉米小麦改良中心(CIMMYT) 的小麦材料进行抗叶锈病基因鉴定,试验结合系谱分析、基因推导和分子标记检测等方法在苗期对36个已知抗病基因载体品种和供试的40份小麦材料接种17个具有毒性差异的叶锈菌生理小种,通过对比供试小麦材料与已知单基因载体品种侵染型,推导出供试小麦材料中可能携带的已知抗叶锈病基因,同时利用12个与已知抗病基因紧密连锁的标记对供试材料进行标记检测,检测结果与基因推导相互验证。进一步将40份供试品系分别种植于河北保定和河南周口试验田,接种叶锈菌混合小种进行田间成株期抗叶锈性鉴定。结果表明,7个供试小麦材料含有Lr1,携带Lr10的有9个品系,携带Lr11Lr34的分别有10个品系,含有Lr14aLr15Lr26的分别有2,4,3个小麦品系;另外,经标记检测成株抗叶锈病基因Lr37Lr46分别存在于22,39个小麦品系中,经田间鉴定有22个小麦品系表现成株抗性。

关键词: 小麦叶锈病, 抗叶锈病基因, 基因推导, 成株抗叶锈性, 分子标记检测