Construction of Drought and Salt Resistance Gene P5CS in Plant Expression Binary Vector

doi:10.7668/hbnxb.2008.06.003

Abstract

Abstract: The gene P5CS (1905 bp) was amplified via PCR using pBI P5CS-F129A plasmid as template and cloned into pBST vector. The Bam HⅠ/ SalⅠfragment from the recombinant plasmid pBST/P5CS was ligated directively between CaMV35S promotor and NOS terminater of the expression vector pCHF3 without GUS gene. The plant expression vector pCHF3/ P5CS was transfered into Agrobacterium tumefaciems strain LBA4404 by freeze-thaw method,and the expression binary plasmid was further identified by PCR. The results showed that pCHF3/P5CS vector was constructed successfully.

Key words: Drought,salt resistance gene, P5CSgene, Plant expression vector

CLC Number:

Q785

CHENG Ji-dong, AN Yu-lin, SUN Rui-fen. Construction of Drought and Salt Resistance Gene P5CS in Plant Expression Binary Vector[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(6): 11-14. doi: 10.7668/hbnxb.2008.06.003.

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