Construction of Recombinant Fowlpox Virus Expressing Chicken Interleukin-18 and Its Biologic Activity Detection

doi:10.7668/hbnxb.2008.06.002

Abstract

Abstract: In order to construct the recombinant fowlpox viruses(rFPV)expressing chicken IL-18, the chicken IL-18 cDNA was cloned into EcoR Ⅰ site of pSY538 plasmid and then subcloned into Sfi Ⅰ site of pSY681 plasmid containing LacZ gene to generate the recombinant plasmid pSY681/ChIL-18.The recombinant plasmid was transfected on the chick- en embryo fibroblasts cell(CEF)that was pre-infected with S-FPV-017.By screening of blue plaques on the CEF overlaid with agar containing X-gal, the rFPV ChIL-18 recombinants were obtained and identified by PCR.Recombinant chicken IL-18 induced in vitro the proliferation of ConA-stimulated chicken splenocytes in MTT assay.

Key words: Chicken interleukin-18 gene, Recombinant fowlpox virus, Construction, Biologic activity

CLC Number:

S82.4
Q786

CHEN Hong-ying, HUANG Qing-yun, CUI Bao-an, LI Xin-sheng, GUAN Qian, LIU Jin-peng. Construction of Recombinant Fowlpox Virus Expressing Chicken Interleukin-18 and Its Biologic Activity Detection[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(6): 6-10. doi: 10.7668/hbnxb.2008.06.002.

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References

[1] Yanagida N,Ogawa R,Li Y,et al.Recombinant fowlpox viruses expressing the glycoprotein B homolog and the pp38 gene of Marek′s disease virus[J].J Virol,1992,66(3):1402-1408.
[2] Heine H G,Boyle D B.Infectious bursal disease virus structural protein VP2 expressed by a fowlpox virus recombinant confers protection against disease in chickens[J].Arch Virol,1993,131(3/4):277-292.
[3] Beard C W,Schnitzlein W M,Tripathy D N.Effect of route of administration on the efficacy of a recombinant fowlpox virus against H5N2 avian influenza[J].Avian Dis,1992,36(4):1052-1055.
[4] Springer W T,Truman R W.Effect of subcutaneous pox vaccination of young chickens on immune response and weight gains[J].Poult Sci,198l,60(6):1213-1220.
[5] Karaca K,Sharma J M,Winslow B J,et al.Recombinant fowlpox virus coexpressing chicken type I IFN and Newcastle disease virus HN and F genes:Influence of IFN on protective efficacy and humoral response of chicken following in ovo or post-hatch administration of recombinant virus[J].Vaccine,1998,16(16):1496-1503.
[6] Okamura H,Tsutsi H,Komatsu T,et al.Cloning of a new cytokine that induces IFN-γ production by T cells[J].Nature,1995,378(6552):88-91.
[7] Gracie J A,Robertson S E,McInnes I B.Interleukin-18[J].J Leukoc Biol,2003,73(2):213-224.
[8] 潘蔚绮,李广兴,刘胜旺.白细胞介素18和鸡白细胞介素18[J].东北农业大学学报,2004,35(1):123-128.
[9] Schneider K,Puehler F,Baeuerle D,et al.cDNA cloning of biologically active chicken interleukin-18[J].J Interferon Cytokine Res,2000,20(10):879-883.
[10] 程相朝,赵德明,吴庭才,等.鸡IL-18真核表达载体的构建及其对新城疫疫苗免疫增强作用的研究[J].畜牧兽医学报,2005,36(5):476-481.
[11] 陈红英,崔保安,黄青云,等.海兰鸡白细胞介素-18全基因的克隆与序列分析[J].中国预防兽医学报,2006,28(1):48-50.
[12] 温纳相,黄青云,陈荣光,等.鸡IL-18基因重组真核表达载体的构建及其表达产物的生物学活性[J].中国兽医科技,2005,35(7):547-550.
[13] Degen W G,Van Zuilekom H I,Soholtes N C,et al.Potentiation of humoral immune responses to vaccine antigens by recombinant chicken IL-18(rChIL-18)[J].Vaccine,2005,23(33):4212-4218.
[14] Lambrecht B,Gonze M,Morales D,et al.Comparison of biological activities of natural and recombinant chicken interferon-gamma[J].Vet Immunol Immunopathol,1999,70(3/4):257-267.
[15] 韩宗玺,刘胜旺,孔宪刚,等.鸡白细胞介素18成熟蛋白在昆虫细胞/杆状病毒系统中的表达[J].中国生物工程杂志,2004,24(4):59-62.

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