Abstract: In order to study the gene function of GO,obtain the transgenic plant,a plant expression vector pBinG O was constructed by recombinating the G lylate oxidase gene fragment from cloning vector pBSSP5 and the binary vector pBin117 at the EcoR Ⅰsite.The fragment of G lylate oxidase gene was inserted between of CaMV 35Spromotor and NOS terminator in pBin117.The identification results showed that the plant expression binary vector pBinG O was constructed successfully.Subequentely GO gene was introduced into apple leaves mediated by Agrobacterium tumerfaciens.Two transgenic resistant plants were proved by PCR preliminarily that the GO gene had integrated into Apples genome.

Key words: Glycolate oxidase gene, Plant expression vector, Agrobacterium tumerfaciens, G enetic transformation, Apple

CLC Number: 

• Q786