Abstract: In this study,the GHfull2length cDNA is cloned with the employment of RT2PCR and RACE2PCR techniques,which is 973 bp in length.The total length cDNA includes an open readingframe (ORF) of 603 bp,5′ UTRof 59 bp and 3′ UTR of 311 bp (Poly A of 25 bp included).Pairwise and multiple alignments of the sequences of ORF,5′ UTR,3′ UTR of Clarias gariepinus GH cDNA with those of Heteropneustes fossilis,Pangasianodon gigas,Silurus meridionalis and Silurus asotus are performed using DNAMAN 5.2.and MEG A 411 software.Sequence alignment analysis indicate that the average homologies are 91.% and 9614 % separately for ORF nucleotide and amino acid sequence between Clarias gariepinus and the 4 species of Siluriforms catfish mentioned above.ORF nucleotide substitution patterns represent the bias of transition between T and C and the average value is 44.5 %.Meanwhile,the base substitutions also show transition/ transversion bias and the average ratio reaches 2.381.5′ UTR and 3′ UTR nucleotide sequence homology are 7514 % and 7710 %,respectively,which are lower conservative than that of ORF nucleotide sequence.

Key words: Clarias gariepinus, Growth hormone, RACE, Full-length cDNA, Sequence analysis

CLC Number: 

• Q785