Construction of Glucose Oxidase Gene in Plant Expression Binary Vector

doi:10.3321/j.issn:1000-7091.2006.06.003

Abstract

Abstract: This is the preparation for genetic transformation.Glucose oxidase gene was cut from pMD/GO with restraction enzymes.A plant expression vector pROK/GO was constructed.The fragment of Glucose oxidase gene in pROKⅡ was directively ligated downstream to CaMV35S promotor and upstream to NOS terminator.The plant expression vector was transformed into A.tumefaciens LBA4404 by the freeze-thaw method.The binary expression plasmid was further identified by PCR and restriction enzymes digestion.The results showed that the plant expression binary vector pROK/GO was constructed successfully.

Key words: Glucose oxidase gene, Plant expression vector

CLC Number:

Q782

SUN Rui-fen, AN Yu-lin, ZHANG He-ling, LAN Hai-ying, WANG Jing, CHENG Ji-dong, LIU Li-ping. Construction of Glucose Oxidase Gene in Plant Expression Binary Vector[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(6): 11-15. doi: 10.3321/j.issn:1000-7091.2006.06.003.

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