Cloning and Plant Expression Vector Construction of Transcription Factor CBF4 Gene in Arabidopsis thaliana

doi:10.7668/hbnxb.2010.04.011

Abstract

Abstract: In order to improve the resistance of alfalfa using CBF4，the experiment has cloned CBF4 genes by PCR technique from the plants of Arabidopsis thaliana and cloned gene sequences of CBF4 had highly homologous with GenBank，reached 99. 41% respectively. Then connected the gene into the plant expression vector PBI121，Basic above all，the experiment has been success constructed the plant expression vector that suitable for the alfalfa agrobacterium genetic transformation. It is a foundation that for next step to using the CBF4 gene fast breeds new high resistance alfalfa varieties.

Key words: CBF4, Arabidopsis thaliana, Clone, Construction of the plant expression vector

CLC Number:

Q78
S637

XU Chun-bo, WANG Yong, ZHAO Hai-xia, LI Xing-you. Cloning and Plant Expression Vector Construction of Transcription Factor CBF4 Gene in Arabidopsis thaliana[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(4): 53-56. doi: 10.7668/hbnxb.2010.04.011.

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http://www.hbnxb.net/EN/Y2010/V25/I4/53

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