Cloning the cD NA of D REB-like Transcription Factor from Chinese Cabbage and Construction of Plant Expression Vector

doi:10.7668/hbnxb.2009.01.016

Abstract

Abstract: The cDNA of DREB transcription factor named BcDRFBl(GenBank accession numberEU924266) was ob-twined from Chinese cabbage inbred line 85-1 under the ought stress by RT-PCR method. The sequence analysis indicatedthat the clone was consisted of 663 nucleotides (nt),coding 214 amino acids. The deduced primary structure of BcDRFBlcontained a silge AP2 cbmain and showed the typical characteristics of DRFB gene family. A plant expression vector of theBcDRFBl gene was constructed and named pCAM BcDRFBl,in which the.S sequence of BcDRFBl was controlled bythe CaMV 35S promoter. This work laid the foundations for future transgenic research on BcDRFBlgene function.

Key words: Chinese cabbage, DRFB transcription factor, Cane cloning, Plant expression vector

CLC Number:

S634.1

LI Yan, SHEN Shu-xing, XUAN Shu-xin, LI Xiao-feng, WANG Yan-hua. Cloning the cD NA of D REB-like Transcription Factor from Chinese Cabbage and Construction of Plant Expression Vector[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(1): 69-73. doi: 10.7668/hbnxb.2009.01.016.

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