Cloning of ETR5 from Uenishiwase Persimmon Fruits and Its Plant Expression Vector Construction

doi:10.7668/hbnxb.2007.04.006

Abstract

Abstract: One fragment of ETR5 was cloned fromUenishiwase persimmon by PCR. Sequence analysis indicated that nucleotide identities of Uenishiwase with Pyrus communis was 79%, and amino acid identities were 81%. Uenishiwase has the conserved amino acid regions and invariant amino acid residues of ETR5 existing in other plants, the cloned production was Uenishiwase persimmon ETR5 gene sequence confirmly. Two pairs of primers containing restriction enzyme site were designed and were used to amplify sequenced plasmid. Two PCR products were digested by the corresponding restricted enzymes respectively, then connect them to be a long segment that is reverse complemented. Finally inserted it into the expression vector pBI221 after digesting.

Key words: Persimmon fruit, Uenishiwase, Ethylene receptor, ETR5, Plant expression vector

CLC Number:

S665.203

CHEN Jia, MA Jun-lian, ZHANG Zi-de, TANG Xia. Cloning of ETR5 from Uenishiwase Persimmon Fruits and Its Plant Expression Vector Construction[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(4): 25-28. doi: 10.7668/hbnxb.2007.04.006.

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