Molecular Cloning and Sequence Analysis of a Phenylalanine Ammonia-lyase cDNA from Nerium oleander

doi:10.7668/hbnxb.2007.04.005

Abstract

Abstract: A phenylalanine ammonia-lyase (PAL) gene was clone from Nerium oleander for the first time and it was also the first cloned gene involved in anthocyanin metabolic pathway in N. oleander. The PAL cDNA fragment of N. oleander (designated as NoPAL1) was 866bp and it encoded a 289 amino acid protein. NoPAL1 was found to have extensive homology with those of other plant PAL genes via multiple alignments. The domination sites and catalytic active sites in PAL protein of Oryza sativa and Zea mays were also found in NoPAL1. Phylogenetic tree analysis revealed that NoPAL1 had closer relationship with PALs from arbor plants than from other plants. NoPAL1 is a useful tool to study the regulation of anthocyanis metabolism in N. oleander.

Key words: NoPAL1, PAL, Nerium oleander, Molecular cloning, Sequence analysis

CLC Number:

S662.103

WANG Yan, XU Feng, DU He-wei, CAI Rong, CHEN Liu-ji, CHENG Shui-yuan. Molecular Cloning and Sequence Analysis of a Phenylalanine Ammonia-lyase cDNA from Nerium oleander[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(4): 19-24. doi: 10.7668/hbnxb.2007.04.005.

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