Cloning and Sequence Analysis of GX Gene of Chicken Infectious Laryngotracheitis Virus Henan Isolate ILTV-CG

doi:10.7668/hbnxb.2007.04.007

Abstract

Abstract: One pair of primers was designed according to the nucleotide sequence of chicken infectious laryngotracheitis virus (ILTV) GX gene from Genbank. The ILTV-CG strain was inoculated into 10-day embryonated eggs, which were used to extract the genome DNA for PCR amplification. The PCR products were purified, and then cloned into pGEM-T Easy vector. By screening of blue-white spot, the plasmid PCR and enzyme digestion, positive strains were sequenced. The results indicated that the amplified GX gene fragment was 950 bp, including an open-reading frame and encoding 299 amino acid residues that contain four potential N-glycosalation sites and eleven cysteines. The sequence analysis results showed a 97.2% similarity between GX gene ILTV-CG and ILTV-SA2 and 95.3% between their putative amino acid sequences. This study paved the way for preparation of recombinant vaccine of ILTV.

Key words: Infectious laryngotracheitis virus, GX gene, Polymerase chain reaction, Sequence analysis

CLC Number:

S432.4+1

CHEN Hong-ying, CUI Bao-an, LI Xin-sheng, YANG Ming-fan, ZHAO Li, FANG Jian-yu. Cloning and Sequence Analysis of GX Gene of Chicken Infectious Laryngotracheitis Virus Henan Isolate ILTV-CG[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(4): 29-32. doi: 10.7668/hbnxb.2007.04.007.

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