Studies on the Expression of Exogenous p5CS Gene in Transgenic Wheatgrasses (Agropyron cristatum×A. Desertorum cv.’ Hycrest-Mengnong’) and Drought Resistance

doi:10.7668/hbnxb.2007.04.008

Abstract

Abstract: In order to breed a new salt tolerance variety which adapt to extend in drought areas in China, The p5CS gene expressed in transgenic hybrid wheatgrass (Agropyron cristatum A. desertorum cv.' Hycrest-Mengnong'), which indentified by PCR analysis and Southern blot, was investigated with Northern blot and physiological index was determined under 1.5% NaCl stress. The results showed that transgenic wheatgrasses and DIG marker hybridize was present obvious strap; under salt stress, Pro contents was increased quickly, cell membrane permeabilities and MDA contents were increased slowly, SOD activity was high. The results indicated that exogenous gene p5CS expressed at transcription level in transgenic plants, and salt tolerance of transgenic plants were enhanced.

Key words: p5CS gene, Northern blot, Salt stress, Salt tolerance

CLC Number:

S53.935.3

WANG Gui-hua, MI Fu-gui, LIU Juan, DONG Shu-jun, HUO Xiu-wen, YANG Hong-yan. Studies on the Expression of Exogenous p5CS Gene in Transgenic Wheatgrasses (Agropyron cristatum×A. Desertorum cv.’ Hycrest-Mengnong’) and Drought Resistance[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(4): 33-36. doi: 10.7668/hbnxb.2007.04.008.

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